| 基本培养基及温度和pH对黄芪组织培养的影响 |
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| 引用本文： | 张延红,高素芳,朱田田,晋玲.基本培养基及温度和pH对黄芪组织培养的影响[J].甘肃农业科技,2011(3):7-9. |
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| 作者姓名： | 张延红 高素芳 朱田田 晋玲 |
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| 作者单位： | 甘肃中医学院药学系,甘肃,兰州,730000 |
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| 基金项目： | 十一五国家科技攻关西部支撑项目"当归、黄芪、大黄种质资源可持续利用关键技术研究"，甘肃省教育厅项目"甘肃道地黄芪工厂化育苗技术研究"，甘肃中医学院教改项目"药用植物组织培养技术实验内容的创新研究" |
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| 摘 要： | 研究了基本培养基、温度及PH对黄芪组织培养的影响.结果表明,最佳初代培养基和培养条件是1/2MS+1.5 mg/L NAA+15 mg/g蔗糖+2 mg/g AC,25℃,pH5.8;最佳继代培养基和培养条件是1/2 MS+0.1 mg/LNAA+30 mg/g蔗糖+2 mg/g AC,20℃,pH5.8.带顶芽的茎段...
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| 关 键 词： | 蒙古黄芪 无菌短枝 培养基 温度 pH |
Effects of Basic Medium,Temperature and pH on Tissue Culture of Astragalus membranaceus Bge. |
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| ZHANG Yan-hong,GAO Su-fang,ZHU Tian-tian,JIN Ling.Effects of Basic Medium,Temperature and pH on Tissue Culture of Astragalus membranaceus Bge.[J].Gansu Agricultural Science and Technology,2011(3):7-9. |
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| Authors: | ZHANG Yan-hong GAO Su-fang ZHU Tian-tian JIN Ling |
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| Institution: | ZHANG Yan-hong,GAO Su-fang,ZHU Tian-tian,JIN Ling(Department of Herbs,Gansu College of TCM,Lanzhou Gansu 730000,China) |
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| Abstract: | The effects of basic medium,temperature and pH on tissue cutture of Astragalus membranaceus Bge.was studied.The results showed that the optimum medium for the primary culture of sterile short shoot is 1/2MS + 1.5 mg/L NAA + 15 mg/g sucrose + 2 mg/g AC;The optimum medium for the subculture is 1/2MS+ 0.1 mg/L NAA+ 30 mg/g sucrose + 2 mg/g AC medium is the best for rooting of sterile short shoot with terminal bud;rooting rate of sterile short shoot with terminal bud is 85% in medium 1/2 MS + 1.5 mg/L NAA +15 m... |
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| Keywords: | pH |
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