首页 | 本学科首页   官方微博 | 高级检索  
     检索      

烟草转GroEL基因抗双生病毒的研究
引用本文:吴丹,张保龙,倪万潮,王荣富,张云华.烟草转GroEL基因抗双生病毒的研究[J].江苏农业学报,2009,25(2).
作者姓名:吴丹  张保龙  倪万潮  王荣富  张云华
作者单位:1. 江苏省农业科学院农业生物技术研究所,江苏南京210014;安徽农业大学生命科学院,安徽合肥230061
2. 江苏省农业科学院农业生物技术研究所,江苏南京,210014
3. 安徽农业大学生命科学院,安徽合肥,230061
摘    要:双生病毒是全球性的重要植物病毒,为提高植物对此病毒的抗性,分离了烟粉虱体内GroEL基因,将其连接到棉花韧皮部特异启动子PGHNBS下游,构建到植物表达载体PBI121上,采用农杆菌介导法转化烟草,获得抗性苗.对抗性植株进行PCR、RT-PCR和病毒侵染检测,结果表明:GroEL基因已经整合到烟草基因组中,并且在转录水平上表达.病毒侵染检测结果显示转基因烟草植株中没有检测到病毒,证明韧皮部特异表达GroEL基因抗双生病毒是可行的.

关 键 词:烟粉虱  双生病毒  GroEL基因  烟草  转化

Study on GroEL Gene against Geminivirus in Tobacco Transformation
WU Dan,ZHANG Bao-long,NI Wan-chao,WANG Rong-fu,ZHANG Yun-hua.Study on GroEL Gene against Geminivirus in Tobacco Transformation[J].Jiangsu Journal of Agricultural Sciences,2009,25(2).
Authors:WU Dan  ZHANG Bao-long  NI Wan-chao  WANG Rong-fu  ZHANG Yun-hua
Institution:1.Institute of Biotechnology;Jiangsu Academy of Agricultural Sciences;Nanjing 210014;China;2.College of Life Sciences;Anhui Agricultural University;Hefei 230061;China
Abstract:The geminivirus is a devastating virus all over the word.In order to develop an effective approach to control this virus,the GroEL gene from Bemisia tabaci which was cloned into PBI121 and drived under phloem specific promoter PGHNBS was isolated.Tobacco plants were transformed by co-cultivating leaves method via Agrobacterium mediation.The regenerated kanamycin resistant tobacco plants were confirmed by PCR,RT-PCR and identification of virus infection.PCR and RT-PCR proved the stable integration of the Gro...
Keywords:Bemisia tabaci  geminivirus  GroEL gene  tobacco  transformation  
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号