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板蓝根多糖的提取及其对猪繁殖与呼吸综合征病毒的体外作用
引用本文:王学兵,崔保安,魏战勇,刘蕊,徐端红,杨明凡.板蓝根多糖的提取及其对猪繁殖与呼吸综合征病毒的体外作用[J].江苏农业学报,2009,25(2).
作者姓名:王学兵  崔保安  魏战勇  刘蕊  徐端红  杨明凡
作者单位:河南农业大学牧医工程学院,河南郑州,450002
基金项目:国家农业科技成果转化资金资助项目 
摘    要:用水煮醇沉法从中药板蓝根中提取板蓝根多糖,作为试验药物,观察其在Marc-145单层细胞上对猪繁殖与呼吸综合征病毒(PRRSV)的作用效果.结果表明,在安全浓度范围内,板蓝根多糖体外对PRRSV具有较好的阻断和抑制作用,最小阻断浓度为2.094 μg/ml,最小抑制浓度为8.375 μg/ml.

关 键 词:板蓝根多糖  细胞病变效应

Extraction of Indigowoad Root Polysaccharides and Its in vitro Reaction to Porcine Reproductive and Respiration Syndrome Virus (PRRSV)
WANG Xue-bing,CUI Bao-an,WEI Zhan-yong,LIU Rui,XU Duan-hong,YANG Ming-fan.Extraction of Indigowoad Root Polysaccharides and Its in vitro Reaction to Porcine Reproductive and Respiration Syndrome Virus (PRRSV)[J].Jiangsu Journal of Agricultural Sciences,2009,25(2).
Authors:WANG Xue-bing  CUI Bao-an  WEI Zhan-yong  LIU Rui  XU Duan-hong  YANG Ming-fan
Institution:College of Animal Husbandry and Veterinary Science;Henan Agricultural University;Zhengzhou 450002;China
Abstract:Indigowoad root polysaccharides was extracted from Radix isatidis by distilled water boiling and alcohol precipitation method and used as the medicine for porcine reproductive and respiration syndrome.The efficacy was indicated by the replication of porcine reproductive and respiration syndrome virus(PRRSV) on the Marc-145 monolayer cell.In the safe concentration scope,indigowoad root polysaccharides has a good in vitro blocking and inhibitory action to PRRSV,the minimum concentration of blocking being 2.09...
Keywords:PRRSV  Marc-145
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