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猪CACNA1S基因部分序列多态性检测及其与肉质性状的关联分析
引用本文:王雪,方晓敏,武玮,刘源,任守文,刘红林.猪CACNA1S基因部分序列多态性检测及其与肉质性状的关联分析[J].江苏农业学报,2010,26(1).
作者姓名:王雪  方晓敏  武玮  刘源  任守文  刘红林
作者单位:1. 江苏省农业科学院畜牧研究所,江苏,南京,210014;南京农业大学动物科学院,江苏,南京,210095
2. 江苏省农业科学院畜牧研究所,江苏,南京,210014
3. 南京农业大学动物科学院,江苏,南京,210095
基金项目:国家资源平台项目(2005DKA2101);;江苏省高新技术研究项目(BG2005305);;江苏省农业科学院博士后基金项目(6510607)
摘    要:采用PCR-SSCP技术,对30头苏钟猪和97头金华与皮特兰杂交F2代(JPF2)猪CACNA1S基因进行多态性检测,结合肉质性状作关联分析。结果表明:在猪CACNA1S基因1 234 bp核酸序列中,检测到25个单核苷酸突变点(SNP);关联分析结果显示,引物1S-65的AA、BB基因型对后腿肌肉重存在显著影响(P<0.05),AB基因型对后腿pH存在显著影响(P<0.05);引物1S-66的BB、BC基因型对蹄重存在极显著影响(P<0.01)。

关 键 词:  CACNA1S基因  PCR-SSCP  肉质性状  相关分析  

Polymorphism of Partial Sequence of the Porcine CACNA1S Gene and Its Association with Meat Quality Traits
WANG Xue,FANG Xiao-min,WU Wei,LIU Yuan,REN Shou-wen,LIU Hong-lin.Polymorphism of Partial Sequence of the Porcine CACNA1S Gene and Its Association with Meat Quality Traits[J].Jiangsu Journal of Agricultural Sciences,2010,26(1).
Authors:WANG Xue    FANG Xiao-min  WU Wei  LIU Yuan  REN Shou-wen  LIU Hong-lin
Institution:1.Institute of Animal Science/a>;Jiangsu Academy of Agricultural Sciences/a>;Nanjing 210014/a>;China/a>;2.College of Animal Science and Technology/a>;Nanjing Agricultural University/a>;Nanjing 210095/a>;China
Abstract:Single-nucleotide polymorphism of CACNA1S gene of 30 Suzhong pigs and 97 F2 offspring of Jinhua and Pietran pigs was analyzed by polymerase chain reaction-single strand conformational polymorphism(PCR-SSCP),and its association analysis with meat quality traits was conducted.In results,25 mutations were found in 1 234 bp of CACNA1S gene.It was observed by association analysis that the AA and BB genotypes of primer 1S-65 had significant effects on ham weight(P<0.05),the AB genotype of primer 1S-65 had signifi...
Keywords:porcine  CACNA1S gene  PCR-SSCP  meat quality trait  association analysis  
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