| 鸡蛋中三聚氰胺残留的检测 |
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| 引用本文： | 魏瑞成,黄思瑜,侯翔,包红朵,陈明,王冉.鸡蛋中三聚氰胺残留的检测[J].江苏农业学报,2008,24(6). |
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| 作者姓名： | 魏瑞成 黄思瑜 侯翔 包红朵 陈明 王冉 |
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| 作者单位： | 江苏省农业科学院食品质量安全与检测研究所,农业部食品安全监控重点开放实验室,江苏省畜禽产品安全性研究重点实验室,江苏,南京,210014 |
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| 基金项目： | 国家“十一五”支撑计划(2006BAK02A28); 江苏省创新专项[CX(08)601] |
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| 摘 要： | 为建立鸡蛋中三聚氰胺残留的简便高效液相色谱法，本试验采用NH2柱作为色谱分析柱，90％乙腈水溶液为流动相，鸡蛋样品经混合均匀后，用三氯乙酸-乙腈溶液提取，经阳离子交换固相萃取柱净化、富集后，在紫外检测器230nm处用高效液相色谱仪测定，保留时间在6～7min。标准曲线在0．1～5．0μg／ml浓度内呈线性相关，相关系数（r）为0．9999。在空白鸡蛋样品中添加0．2mg／kg、0．5mg／kg、2．0mg/kg和5．0mg／kg的三聚氰胺，回收率为78．96％～92．19％，相对标准偏差（RSD）为1．96％～6．60％。样品检测定量限为0．2mng／kg。
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| 关 键 词： | 鸡蛋 三聚氰胺 残留 高效液相色谱法 |
Determination of Melamine Residues in Egg |
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| WEI Rui-cheng,HUANG Si-yu,HOU Xiang,BAO Hong-duo,CHEN Ming,WANG Ran.Determination of Melamine Residues in Egg[J].Jiangsu Journal of Agricultural Sciences,2008,24(6). |
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| Authors: | WEI Rui-cheng HUANG Si-yu HOU Xiang BAO Hong-duo CHEN Ming WANG Ran |
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| Abstract: | A high-performance liquid chromatography(HPLC) with NH2 column and 90% acetonitrile eluate to determine the melamine in eggs was developed.Samples were extracted with 1% trichloroacetic acid-acetonitrile.Target analytes of samples were cleaned up and concentrated by HyperSep Retain-CX column solid-phase extraction(SPE).A perfect separation for melamine was achieved and retention time was about 6-7 min at 230 nm.The calibration curve was linear in a concentration range of 0.1-5.0 g/ml,with correlation coeffi... |
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| Keywords: | egg melamine residue high-performance liquid chromatography |
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