|
|||||
|
|
| 辣椒抗黄瓜花叶病毒同源序列的克隆与分析 | |
| 引用本文： | 杨学玲,王述彬,刘金兵,潘宝贵,万红建,陈劲枫.辣椒抗黄瓜花叶病毒同源序列的克隆与分析[J].江苏农业学报,2009,25(4). |
| 作者姓名： | 杨学玲 王述彬 刘金兵 潘宝贵 万红建 陈劲枫 |
| 作者单位： | 1. 南京农业大学园艺学院,江苏,南京,210095;江苏省农业科学院蔬菜研究所,江苏,南京,210014 2. 江苏省农业科学院蔬菜研究所,江苏,南京,210014 3. 南京农业大学园艺学院,江苏,南京,210095 |
| 基金项目： | 国家"863"计划，国家"十一五"科技支撑项目，江苏省农业科技自主创新资金项目，江苏省科技基础设施建设计划 |
| 摘 要： | 根据已知抗病基因的核苷酸结合位点(NBS)保守结构域设计简并引物,以高抗黄瓜花叶病毒的辣椒Yv2007001为试材,获得10条抗病基因同源序列(RGA).经序列分析,这些RGAs皆具有开放阅读框和NBS保守结构域(P-loop、Kinase-2、Kinase-3a、RNBS-C和GLPL),属于nonTIR-NBS-LRR类抗病基因同源序列.其中RGA46与番茄花叶病毒抗性基因Tm-22的氨基酸同源性为78%,可能与辣椒抗黄瓜花叶病毒(CMV)相关.其它序列与已知抗病基因Prf、RPP13、I2C-1、Mi-1.2、L6、M氨基酸相似性为21%～70%.所得RGA序列的Ka/Ks值为0.011 0～0.305 1,均显著小于1,表明"纯化选择"在辣椒NBS区域进化中起主要作用. |
| 关 键 词： | 辣椒 黄瓜花叶病毒 抗病基因同源序列 核苷酸结合位点-富含亮氨酸重复 |
Analysis and Cloning of Cucumber Mosaic Virus Resistance Gene Analogs in Pepper(Capsicum annuum L.) |
|
| YANG Xue-ling,WANG Shu-bin,LIU Jin-bing,PAN Bao-gui,WAN Hong-jian,CHEN Jing-feng.Analysis and Cloning of Cucumber Mosaic Virus Resistance Gene Analogs in Pepper(Capsicum annuum L.)[J].Jiangsu Journal of Agricultural Sciences,2009,25(4). | |
| Authors: | YANG Xue-ling WANG Shu-bin LIU Jin-bing PAN Bao-gui WAN Hong-jian CHEN Jing-feng |
| Institution: | 1.College of Horticulture;Nanjing Agricultural University;Nanjing 210095;China;2.Institute of Vegetable Crops;Jiangsu Academy of Agricultural Sciences;Nanjing 210014;China |
| Abstract: | Ten resistance gene analogs(RGAs) were cloned from a pepper variety(Yv2007001)with high resistance to cucumber mosaic virus(CMV),using a PCR approach with degenerate primers designed from conserved motifs found in a number of plant resistance genes.Sequence analysis showed that all RGAs belong to nonTIR-NBS-LRR subgroup,containing ORF and five conserved motifs including P-loop,kinase-2a,kinase-3a,RNBS-C and GLPL.RGA46 showed 78% homology at the deduced amino acid with Tm-22(ToMV),suggesting that RGA46 has c... |
| Keywords: | |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |