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| 猪CACNA1S基因的PCR-SSCP检测及其对肉质性状的遗传效应分析 | |
| 引用本文： | 方晓敏,徐宁迎,任守文.猪CACNA1S基因的PCR-SSCP检测及其对肉质性状的遗传效应分析[J].江苏农业学报,2009,25(2). |
| 作者姓名： | 方晓敏 徐宁迎 任守文 |
| 作者单位： | 1. 江苏省农业科学院畜牧研究所,江苏南京210014;浙江大学动物科学院,浙江杭州310029 2. 浙江大学动物科学院,浙江杭州,310029 3. 江苏省农业科学院畜牧研究所,江苏南京,210014 |
| 基金项目： | 国家自然科学基金，国家重大基础规划项目 |
| 摘 要： | 应用PCR-SSCP技术检测126头金华和皮特兰猪杂交产生的金皮F2代试验猪群CACNA1S基因的单核苷酸多态性,结合屠宰测定的肉质及屠体性状指标作遗传效应分析.结果表明:位点1S-46的BC、BD基因型对系水力存在极显著影响(P＜0.01),BC基因型对肉色(L) 存在显著影响(P＜0.05),BB基因型对眼肌面积存在显著影响(P＜0.05);位点1S-61的BC基因型、1S-70的AA基因型和1S-72的AA基因型对后腿重均存在极显著或显著影响(分别为P＜0.01,P＜0.05 和 P＜0.01);位点1S-64的BB基因型对后腿温度存在极显著影响(P＜0.01),对眼肌温度存在显著影响(P＜0.05);位点1S-74的AB、AC、AE基因型分别对后腿重、背膘厚(中)以及系水力存在极显著影响(均为P＜0.01). |
| 关 键 词： | 猪 肉质性状 |
Association of CACNA1S Gene with Carcass and Meat Quality Traits in F2 of Jinhua × Pietrain Swine |
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| FANG Xiao-min,XU Ning-ying,REN Shou-wen.Association of CACNA1S Gene with Carcass and Meat Quality Traits in F2 of Jinhua × Pietrain Swine[J].Jiangsu Journal of Agricultural Sciences,2009,25(2). | |
| Authors: | FANG Xiao-min XU Ning-ying REN Shou-wen |
| Institution: | 1.Institute of Animal Husbandry;Jiangsu Academy of Agricultural Sciences;Nanjing 210014;China;2.College of Animal Science;Zhejiang University;Hangzhou 310029;China |
| Abstract: | Single-nucleotide polymorphism of CACNA1S gene was detected by polymerase chain reaction-single strand conformational polymorphism(PCR-SSCP) in 126 crossbred of Jinhua and Pietrain,and the genetic effects of CACNA1S gene on the carcass and meat quality traits were analyzed.The results indicated that both the genotypes BC and BD of the locus 1S-46 had significant effects on water-holding capacity(P<0.01),BC had significant effect on meat lightness(L)(P<0.05) and BB had significant effect on eye muscle area(P... |
| Keywords: | CACNA1S PCR-SSCP |
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