| 美国甘薯育种材料遗传多样性的ISSR分析 |
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| 引用本文： | 吴觐宇,傅玉凡,张华玲,张启堂,GEORGE CRAIG Y.美国甘薯育种材料遗传多样性的ISSR分析[J].江苏农业学报,2009,25(6). |
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| 作者姓名： | 吴觐宇 傅玉凡 张华玲 张启堂 GEORGE CRAIG Y |
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| 作者单位： | 1. 西南大学生命科学学院,三峡库区生态环境教育部重点实验室,重庆市甘薯工程技术研究中心,重庆,400715
2. Depart-ment of Horticultural Science,North Carolina State University,Raleigh,NC 27695-7609,USA |
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| 基金项目： | 农业部"948"项目 |
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| 摘 要： | 对25份美国甘薯Ipomoea batatas (L.) Lam.]品系及另外作为对照的47份甘薯种质资源进行ISSR分析,利用NTSYS软件分析遗传相似系数,采用UPGMA方法进行聚类,构建亲缘关系系统图.结果显示:10个引物共扩增出78个条带,其中有73条呈现多态性,多态位点比例高达93.6%;25份美国甘薯育种材料与对照种质资源的平均遗传相似性系数是0.74.表明美国甘薯育种材料遗传多样性较高,可将其中的6-3-1、6-8-7等品系应用于国内甘薯育种中,以扩大遗传背景.
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| 关 键 词： | 甘薯 种质资源 亲缘关系 |
ISSR Marker Diversity with Sweetpotato Lines Collected from USA |
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| WU Jin-yu,FU Yu-fan,ZHANG Hua-ling,ZHANG Qi-tang,GEORGE CRAIG Y.ISSR Marker Diversity with Sweetpotato Lines Collected from USA[J].Jiangsu Journal of Agricultural Sciences,2009,25(6). |
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| Authors: | WU Jin-yu FU Yu-fan ZHANG Hua-ling ZHANG Qi-tang GEORGE CRAIG Y |
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| Abstract: | To understand the genetic diversity of sweetpotato (Ipomoea batatas) collected from USA, 72 sweetpota-to breeding lines were analyzed using ISSR markers. The genetic similarity of genetic relationship was calculated by NTSYS software, and dendrogram was clustered by UPGMA method. Based on 73 polymorphic bands amplified from ten primer pairs, genetic similarities among 25 USA lines and 47 control lines ranged from 0. 52 to 0. 94, with an average genetic simi-larity of 0. 74. The results showed that sufficient polymorphism could be found among USA sweetpotato breeding lines, and some lines, such as 6-3-1 and 6-8-7 , could be potential for sweetpotato hybridization breeding. |
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| Keywords: | ISSR sweetpotato ISSR marker genetic diversity |
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