病毒性出血症疫苗免疫剂量对幼兔血清抗体效价的影响

选择35、40和45日龄的新西兰家兔各30只,每种日龄兔随机均分为5组,其中对照组注射生理盐水,4个试验组分别注射4种剂量(1 m l、2 m l、3 m l、4 m l)的兔病毒性出血症疫苗,分别于免疫前及免疫后1~7周内每周采血测定血清抗体效价的动态变化。结果表明:各日龄兔的2 m l、3 m l、4 m l剂量组的抗体上升速度和有效抗体维持时间均显著大于同日龄的1 m l剂量组;35日龄兔的2 m l、3 m l、4 m l剂量组的抗体峰值显著高于1 m l剂量组。提示35日龄兔注射剂量2 m l为最佳免疫程序。

Effect of Dosage of Rabbit Hemorrhagic Disease Vaccine on Serum Antibody Titer in Young Rabbit

35-day old,40-day old and 45-day old young New Zealand rabbits,30 per age,were chosen and divided randomly into five equal groups.The rabbits in control group were injected with physiological saline,in 4 trial groups,vaccinated with four dosages of rabbit hemorrhagic disease(RHD) vaccine respectively.The antibody titer before and 1 to 7 weeks after vaccination were measured.The antibody rising speed and duration of effective antibody in the rabbit groups vaccinated with 2 ml,3 ml and 4 ml of RHD vaccination were significantly more than those in the same age rabbits group injected with 1 ml of RHD vaccine.Antibody peak in the 35-day old rabbit group vaccinated with 1 ml of RHD vaccine was significantly lower than that in other groups.It was suggested that the best immune program be 35 day-old rabbit vaccinated with 2 ml of RHD vaccine.