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玉米杂交种苏玉16农艺性状的QTL分析
引用本文:汤在祥,贾波,张丹,陆鑫,邓德祥,徐辰武.玉米杂交种苏玉16农艺性状的QTL分析[J].江苏农业学报,2012,28(2):265-273.
作者姓名:汤在祥  贾波  张丹  陆鑫  邓德祥  徐辰武
作者单位:1. 扬州大学,江苏省作物遗传生理重点实验室,教育部植物功能基因组学重点实验室,江苏扬州225009;苏州大学,苏州大学医学部公共卫生学院,江苏苏州215123
2. 扬州大学,江苏省作物遗传生理重点实验室,教育部植物功能基因组学重点实验室,江苏扬州225009
基金项目:国家重大基础研究发展规划(973计划)项目,国家自然科学基金,江苏省高校自然科学基金重点项目
摘    要:采用强优势玉米杂交种苏玉16(JB×Y53)的两个亲本自交系,配置F2和相应F2∶3作图群体。利用154个SSR标记构建了分子标记连锁图谱,覆盖全基因组1 735.0 cM,标记间平均图距为11.3 cM。同时考察F2和F2∶3群体的株高、穗位高、抽穗期和散粉期等共10个重要农艺性状,采用联合F2和F2∶3群体的作图方法定位有关QTL。此外,采用QTL Cartographer V2.5软件分别对F2和F2∶3群体进行了有关QTL的重演性验证。结果表明,采用联合作图方法在调查的10个性状上共定位到93个QTL,采用QTL Cartographer V2.5软件共定位到96个QTL,其中56个能用两种方法重演验证。

关 键 词:玉米  数量性状基因座位  农艺性状  联合作图

QTL mapping for agronomic traits of maize hybrid Suyu 16
TANG Zai-xiang , JIA Bo , ZHANG Dan , LU Xin , DENG De-xiang , XU Chen-wu.QTL mapping for agronomic traits of maize hybrid Suyu 16[J].Jiangsu Journal of Agricultural Sciences,2012,28(2):265-273.
Authors:TANG Zai-xiang  JIA Bo  ZHANG Dan  LU Xin  DENG De-xiang  XU Chen-wu
Institution:1(1.Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology,Key Laboratory of Plant Functional Genomics of Ministry of Education,Yangzhou University,Yangzhou 225009,China;2.School of Public Health,Medical College of Soochow University,Suzhou 215123,China)
Abstract:The maize inbred lines JB and Y53,the parental lines of maize hybrid Suyu 16,were used to create F2 and F2∶3 segregation populations.The genetic linkage map was constructed containing 154 SSR markers,which covered 1 735.0 cM of maize genome.The average distance of the flanking markers was 11.3 cM.The phenotypic value of 10 traits including plant height,ear height,heading date,pollen shedding,etc were investigated in F2 and F2∶3 populations.The QTL associated with these traits were mapped through jointing F2 and F2∶3 populations.The composite interval mapping(CIM) in software QTL Cartographer V2.5 was also used to map the QTL in F2 and F2∶3 populations,respectively.93 QTLs were identified in the 10 traits by joint mapping method,and 96 QTLs were identified by CIM method.56 QTLs among 93 QTLs could be verified by CIM method.
Keywords:maize  quantitative trait locus  agronomic trait  joint mapping
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