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| 枯萎病菌对节瓜抗镰刀菌酸细胞系叶片中生化指标的影响 | |
| 引用本文： | 王永飞,王力先,马三梅,何晓明.枯萎病菌对节瓜抗镰刀菌酸细胞系叶片中生化指标的影响[J].江苏农业学报,2012,28(2):402-405. |
| 作者姓名： | 王永飞 王力先 马三梅 何晓明 |
| 作者单位： | 1. 暨南大学生物工程学系,广东广州,510632 2. 暨南大学生物工程学系,广东广州510632;广东省农业科学院蔬菜研究所,广东广州510640;广东省体育科学研究所,广东广州510663 3. 广东省农业科学院蔬菜研究所,广东广州,510640 |
| 基金项目： | 国家自然科学基金，广东省农业攻关重大专项基金 |
| 摘 要： | 为了探讨节瓜抗镰刀菌酸细胞系抗枯萎病的机理,本试验测定了接种枯萎病菌后节瓜抗镰刀菌酸细胞系、抗枯萎病品系和感病品系叶片中过氧化物酶(POD)活性、多酚氧化酶(PPO)活性和可溶性蛋白含量。结果显示,接种枯萎病菌后,枯萎病感病品系、抗镰刀菌酸细胞系、抗枯萎病品系的节瓜叶片POD、PPO活性均极显著高于未接种病菌的对照(P<0.01),而3个材料的可溶性蛋白质含量均极显著低于未接种病菌的对照(P<0.01)。表明通过离体筛选获得的抗镰刀菌酸细胞系与传统抗病材料具有相似的枯萎病抗机理。 |
| 关 键 词： | 节瓜 枯萎病菌 抗镰刀菌酸细胞系 抗病性 |
Effects of Fusarium oxysporum on biochemical indicators in fusaric acid resistant cell line leaves of Chieh-qua (Benincasa hispida Cogn.var.Chieh-qua How.) |
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| WANG Yong-fei , WANG Li-xian , MA San-mei , HE Xiao-ming.Effects of Fusarium oxysporum on biochemical indicators in fusaric acid resistant cell line leaves of Chieh-qua (Benincasa hispida Cogn.var.Chieh-qua How.)[J].Jiangsu Journal of Agricultural Sciences,2012,28(2):402-405. | |
| Authors: | WANG Yong-fei WANG Li-xian MA San-mei HE Xiao-ming |
| Institution: | 1.Department of Biotechnology,Jinan University,Guangzhou 510632,China;2.Vegetable Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China;3.Guangdong Research Institute of Sports Science,Guangzhou 510663,China) |
| Abstract: | To investigate the resistant mechanism of fusaric acid cell line of Chieh-qua to fusarium wilt,the activities of peroxidase(POD) and polyphenoloxidase(PPO),and the content of soluble protein in fusaric acid resistant cell line,resistant line and sensitive line leaves of Chieh-qua inoculated with Fusarium oxysporum were determined.After inoculation with Fusarium oxysporum,POD and PPO activities in fusaric acid resistant cell line,resistant line and sensitive line leaves of Chieh-qua were significantly higher than those in control(P<0.01),while the content of soluble protein in three materials were significantly lower than that in control(P<0.01).The results indicated that the resistant mechanism to Fusarium oxysporum in fusaric acid resistant cell line selected in vitro were similar to that in resistant lines obtained through traditional way. |
| Keywords: | Chieh-qua(Benincasa hispida Cogn var Chieh-qua How ) Fusarium oxysporum fusaric acid resistant cell line disease resistance |
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