江苏省稻曲病菌的群体遗传结构

为了分析来源于江苏省徐州市、苏州市、南京市、盐城市和南通市的48个稻区稻曲病菌Ustilaginoideavirens(Cook.)Tak.]的群体遗传多样性,通过Rep-PCR技术,用3对特异性引物BOX、REP和ERIC对48个菌株基因组DNA进行了PCR扩增,以彼此间的带位相似率达90%为界,BOX扩增的谱型被分为4簇,REP的谱型被分为4簇,ERIC的谱型被分为5簇。48个稻曲病菌株接种于感病品种两优培九上,被划分为3个致病力类型,其中弱致病性类型占50%,为优势致病性类型。由于参试菌株遗传多样性值最大(0.55),簇群分类与地理位置显著相关,所以BOX引物较其他两种引物更适合进行稻曲病菌的群体遗传多样性分析。群体遗传多样性值BOX为0.55,REP为0.44,ERIC为0.42,说明江苏省这5个地区稻曲病菌群体的遗传分化不明显,遗传多样性稳定。江苏省5大稻区48个稻曲病菌的BOX、REP、ERIC簇群分类与地理位置、致病性类型均无显著的相关性。

Genetic diversity of Ustilaginoidea viren from rice in Jiangsu province

Forty eight isolates of Ustilaginoidea viren were collected from Xuzhou City,Suzhou City,Nanjing City,Yancheng City and Nantong City,Jiangsu Province,and their genetic diversities and pathotype variabilities were analyzed by using Rep-PCR techniques.Genomic DNA from 48 strains was amplified with three specific primers of BOX,REP and ERIC.Dendrograms were generated from the data by using UPGMA analysis.The strains tested were classed into 4 clusters,4 clusters and 5 clusters based on the PCR results of BOX,REP and ERIC at the level of 90% similarity,respectively.Forty eight isolates were inoculated on susceptible rice cultivar,Liangyoupeijiu.The isolates were divided into three pathotypes,among which weak pathotype(occupied more than 50%) was predominant.Because genetic diversity of strains was maximum(0.55) and classification of cluster group was significantly associated with the origin of isolates,primer BOX was more suitable for the analysis of genetic diversity of U.virens population than the other two primers.The genetic diversities of the U.virens population were 0.54(in BOX),0.44(in REP) and 0.47(in ERIC),respectively.The results indicated that the genetic diversity of U.virens was not obvious.There was no significant correlation between UPGMA groups(BOX,REP and ERIC clusters) of the 48 isolates of U.virens in 5 rice regions in Jiangsu province and the pathotypes and the origin.