| 用花粉管通道法将慈姑蛋白酶抑制剂基因(API)导入水稻获得转基因植株 |
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| 引用本文： | 王才林,朱镇,赵凌,张亚东,林静,张所兵,陈涛,刘贤进,王冬兰,黄骏麒,龚蓁蓁.用花粉管通道法将慈姑蛋白酶抑制剂基因(API)导入水稻获得转基因植株[J].江苏农业学报,2005,21(4):245-248. |
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| 作者姓名： | 王才林 朱镇 赵凌 张亚东 林静 张所兵 陈涛 刘贤进 王冬兰 黄骏麒 龚蓁蓁 |
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| 作者单位： | 1. 江苏省农业科学院粮食作物研究所暨江苏省优质水稻工程技术研究中心,江苏,南京,210014
2. 中国科学院上海生物化学研究所,上海,200031 |
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| 基金项目： | 江苏省高技术研究项目(BG2001304,BG2002301) |
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| 摘 要： | 采用花粉管通道法遗传转化技术,将慈姑蛋白酶抑制剂基因(API)导入水稻品系9311中,获得了转基因植株。抗虫性鉴定结果表明:部分转基因植株对水稻螟虫的抗性比对照明显提高;PCR扩增获得了与引物扩增片段长度相同的DNA片段,证实API基因已整合到受体植株的基因组中。遗传分析表明,API基因能在有性生殖过程中传递给后代,并于T4代分离出抗性纯合的株系。
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| 关 键 词： | 水稻 抗虫 慈姑蛋白酶抑制剂基因 转基因植株 花粉管通道法 |
| 文章编号： | 1000-4440（2005）04-0245-04 |
| 收稿时间： | 2005-05-17 |
| 修稿时间： | 2005年5月17日 |
Transgenic Rice Plant Resulting from Introduction of Arrowhead Proteinase Inhibitor Gene (API) into Rice by the Pollen-tube Pathway Method |
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| WANG Cai-lin,ZHU Zhen,ZHAO Ling,ZHANG Ya-dong,LIN Jing,ZHANG Suo-bing,CHEN Tao,LIU Xian-jin,WANG Dong-lan,HUANG Jun-qi,GONG Zhen-zhen.Transgenic Rice Plant Resulting from Introduction of Arrowhead Proteinase Inhibitor Gene (API) into Rice by the Pollen-tube Pathway Method[J].Jiangsu Journal of Agricultural Sciences,2005,21(4):245-248. |
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| Authors: | WANG Cai-lin ZHU Zhen ZHAO Ling ZHANG Ya-dong LIN Jing ZHANG Suo-bing CHEN Tao LIU Xian-jin WANG Dong-lan HUANG Jun-qi GONG Zhen-zhen |
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| Abstract: | Transgenic rice plants were obtained by transferring the arrowhead proteinase inhibitor gene(API) into rice line 9311 through the pollen-tube pathway method.Some transgenic rice plants were proved to have expressed better resistance to insect pest than the check line 9311.Analysis of PCR showed the integration of API gene into the genome of the transgenic plant.Genetic analysis indicated that the API gene was genetically delivered to the progeny and homozygous lines with insect resistance appeared in T_4. |
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| Keywords: | rice(Oryza sativa L ) insect pest resistance API gene transgenic plant pollen-tube pathway method |
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