| 慈姑蛋白酶抑制剂(API)基因导入棉花获得转基因植株 |
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| 引用本文： | 黄骏麒,龚蓁蓁,吴敬音,陈松,束春娥,谢伟军,周宝良,沈新莲.慈姑蛋白酶抑制剂(API)基因导入棉花获得转基因植株[J].江苏农业学报,2001,17(2). |
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| 作者姓名： | 黄骏麒 龚蓁蓁 吴敬音 陈松 束春娥 谢伟军 周宝良 沈新莲 |
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| 基金项目： | 江苏省农业科技攻关项目 |
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| 摘 要： | 通过花粉管通道技术将慈姑蛋白酶抑制剂API(ArrowheadProteinaseInhibitor)基因转入棉花 ,获得了转基因植株。经对棉铃虫抗虫性鉴定和PCR、RT PCR、Southernblotting分析 ,证明获得了表达API基因的抗虫棉
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| 关 键 词： | 慈姑蛋白酶抑制剂API基因 棉花 转基因植株 |
Transgenic Cotton Plant Resulting from Introduction of Arrowhead Proteinase Inhibitor (API) Gene into Cotton |
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| HUANG Junqi,GONG Zhenzhen,WU Jingyin,CHEN Song,SHU Chune,XIE Weijun,ZHOU Baoliang,SHEN Xinlian.Transgenic Cotton Plant Resulting from Introduction of Arrowhead Proteinase Inhibitor (API) Gene into Cotton[J].Jiangsu Journal of Agricultural Sciences,2001,17(2). |
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| Authors: | HUANG Junqi GONG Zhenzhen WU Jingyin CHEN Song SHU Chune XIE Weijun ZHOU Baoliang SHEN Xinlian |
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| Institution: | WT5BZ] 1HUANG Junqi,2GONG Zhenzhen,3WU Jingyin,1CHEN Song,4SHU Chune,2XIE Weijun,1ZHOU Baoliang,1SHEN Xinlian |
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| Abstract: | The gene for arrowhead proteinase inhibitor（API） was transferred into cotton plants by the pollen-tube pathway method，and transgenic cotton plant was resulted．After tests of resistance to pest insects and analyses of PCR，RT-PCR and Southern blotting，the transgenic plants were proved to have expressed the API gene and to be resistant to cotton bollworm．This is the first report so far concerning the pest-resistant transgenic cotton with the API gene． |
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| Keywords: | arrowhead proteinase inhibiter gene(API) cotton transgenic plant |
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