| 玉米PEPC基因和抗白叶枯病基因Xa25的水稻聚合育种 |
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| 引用本文： | 高东迎,郭士伟,何冰,张彦,焦德茂.玉米PEPC基因和抗白叶枯病基因Xa25的水稻聚合育种[J].江苏农业学报,2006,22(1):5-9. |
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| 作者姓名： | 高东迎 郭士伟 何冰 张彦 焦德茂 |
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| 作者单位： | 江苏省农业科学院农业生物遗传生理研究所,江苏,南京,210014 |
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| 摘 要： | 利用转玉米PEPC基因水稻HPTER(高感水稻白叶枯病)和抗白叶枯病水稻HX-3(具有抗白叶枯病基因Xa25)杂交,获得F1杂种,并对F1杂种进行花药培养,获得42株二倍体植株。通过对这些植株后代进行抗白叶枯病性鉴定,PEPC酶活性和光合速率测定及PCR检测,最后获得同时具有玉米PEPC基因和抗白叶枯病基因Xa25的水稻聚合株系7个,这些株系的农艺性状较HPTER有较大改善,可作为培育高产、抗白叶枯病水稻新品种的种质资源。
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| 关 键 词： | 水稻 PEPC基因 抗白叶枯病基因Xa25 聚合 |
| 文章编号： | 1000-4440（2006）01-0005-05 |
| 收稿时间： | 2005-07-14 |
| 修稿时间： | 2005年7月14日 |
Pyramiding of Maize PEPC Gene and Bacterial Blight Resistance Gene Xa25 in Rice |
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| GAO Dong-ying,GUO Shi-wei,HE Bing,ZHANG Yan,JIAO De-mao.Pyramiding of Maize PEPC Gene and Bacterial Blight Resistance Gene Xa25 in Rice[J].Jiangsu Journal of Agricultural Sciences,2006,22(1):5-9. |
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| Authors: | GAO Dong-ying GUO Shi-wei HE Bing ZHANG Yan JIAO De-mao |
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| Institution: | Institute of Agribiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China |
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| Abstract: | A transgenic rice cultivar HPTER,which expresses a maize phosphoenolpyruvate carboxylase(PEPC)gene and is highly susceptible to bacteria blight disease(BB),was crossed with a BB resistant cultivar HX-3 containing a resistance gene Xa25.By anther culture from the F_1 hybrids of the cross,a total of 42 doubled-haploid(DH) regenerated plants were obtained.BB resistance evaluation,PCR analysis and detection of PEPC activity and photosynthetic rate in the DH line led to the identification of 7 pyramid DH lines containing both PEPC gene and Xa25 gene.The pyramid DH lines,with their agronomic traits greatly improved,may act as valuable germplasms for the development of new disease-resistant high-yielding rice varieties. |
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| Keywords: | rice PEPC gene bacterial blight resistance gene Xa25 pyramiding |
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