| 贯叶金丝桃的电子束辐照杀菌研究 |
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| 引用本文： | 靳振召,梁剑平,王学红,刘宇,华兰英.贯叶金丝桃的电子束辐照杀菌研究[J].江苏农业学报,2010,26(1). |
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| 作者姓名： | 靳振召 梁剑平 王学红 刘宇 华兰英 |
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| 作者单位： | 1. 甘肃农业大学动物医学院,甘肃,兰州,730070
2. 中国农业科学院兰州畜牧与兽药研究所新兽药工程重点实验室,甘肃,兰州,730050 |
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| 基金项目： | 国家“十一五”科技支撑计划(2006BAD31B05);;中国科学院“百人计划”(2008-287) |
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| 摘 要： | 研究电子束辐照贯叶金丝桃杀菌的可行性,为科研和生产中有效地应用该方法提供理论依据。辐照样品中微生物数量的测定依据《中华人民共和国药典》提供的方法;应用高效液相色谱检测有效成分金丝桃素和金丝桃苷含量;依据微生物存活对数与辐照剂量的线性关系,求得D10;采用目测、鼻嗅、品尝的评定方法,评定样品辐照前后的外部性状变化。结果表明:细菌、霉菌+酵母菌的D10值分别为2.91 kGy和4.69 kGy,霉菌和酵母菌相对于细菌对电子束有更强的耐受力,适宜的辐照加工剂量为4.5~6.5 kGy,经过辐照后,样品可以保存30~150 d,卫生指标仍达到国家药典中规定的中药粉剂微生物限度标准,金丝桃苷和金丝桃素的含量未发生显著变化。因此,利用电子束辐照贯叶金丝桃杀菌是一种行之有效的方法。
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| 关 键 词： | 电子束辐照 杀菌 贯叶金丝桃 菌落数量 金丝桃素 金丝桃苷 |
Decontamination of Hypericum performatum L. by Electron-beam Irradiation |
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| JIN Zhen-zhao,LIANG Jian-ping,WANG Xue-hong,LIU Yu ,HUA Lan-ying.Decontamination of Hypericum performatum L. by Electron-beam Irradiation[J].Jiangsu Journal of Agricultural Sciences,2010,26(1). |
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| Authors: | JIN Zhen-zhao LIANG Jian-ping WANG Xue-hong LIU Yu HUA Lan-ying |
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| Institution: | 1.College of Veterinary Medicine/a>;Gansu Agricultural University/a>;Lanzhou 730070/a>;China/a>;2.Lanzhou Institute of Animal Husbandry and Veterinary Pharmacology/a>;Chinese Academy of Agricultural Sciences/a>;Lanzhou 730050/a>;China |
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| Abstract: | To address the feasibility to decontaminate Hypericum perforatum L.irradiated by electron beam,microbial number was counted based on Pharmacopoeia of the People's Republic of China,and high performance liquid chromatography was applied for determining the contents of hypericin and hyperin in Hypericum performatum L.The measured data was analyzed by one-dimensional analysis of variance(SPSS),and the D10values were calculated on the basis of the linear relationship between the logarithmic value of surviving m... |
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| Keywords: | electron-beam irradiation decontamination Hypericum perforatum L number of colony hypericin hyperin |
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