| 嗜水气单胞菌Aer毒素和胞外蛋白酶卵黄抗体的制备 |
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| 引用本文： | 王帅兵,熊良伟,朱善元,于生兰,徐建生,吴海波.嗜水气单胞菌Aer毒素和胞外蛋白酶卵黄抗体的制备[J].江苏农业学报,2012(1):135-139. |
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| 作者姓名： | 王帅兵 熊良伟 朱善元 于生兰 徐建生 吴海波 |
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| 作者单位： | 江苏畜牧兽医职业技术学院;江苏省兽用生物制药高技术研究重点实验室;扬州大学兽医学院 |
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| 基金项目： | 江苏畜牧兽医职业技术学院2009年院级重点课题(ZD200907) |
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| 摘 要： | 为克服嗜水气单胞菌不同血清型对卵黄抗体免疫保护效果的影响,从嗜水气单胞菌中提取其主要致病因子Aer毒素和胞外蛋白酶(ECPase),验证其生物学活性,分别免疫蛋鸡。用辛酸-硫酸铵法粗提卵黄抗体,用间接ELISA法测其效价、鉴定抗体与不同来源嗜水气单胞菌主要致病因子的交叉反应。结果表明,抗Aer毒素和抗ECPase卵黄抗体效价最高分别可达1∶213和1∶212,与其他来源嗜水气单胞菌交叉反应性强。
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| 关 键 词： | 嗜水气单胞菌 Aer毒素 胞外蛋白酶 卵黄抗体 交叉反应性 |
Preparation of chicken egg yolk immunoglobulin containing antibody against Aer toxins and ECPases in Aeromonas hydrophila |
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| WANG Shuai-bing,XIONG Liang-wei,ZHU Shan-yuan,YU Sheng-lan,XU Jian-sheng,WU Hai-bo.Preparation of chicken egg yolk immunoglobulin containing antibody against Aer toxins and ECPases in Aeromonas hydrophila[J].Jiangsu Journal of Agricultural Sciences,2012(1):135-139. |
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| Authors: | WANG Shuai-bing XIONG Liang-wei ZHU Shan-yuan YU Sheng-lan XU Jian-sheng WU Hai-bo |
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| Institution: | 1.Jiangsu Animal Husbandry and Veterinary College,Taizhou 225300,China;2.Jiangsu Provincial Key Laboratory of Veterinary Bio-pharmaceutical High-tech Research,Taizhou 225300,China;3.College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China) |
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| Abstract: | In order to avoid the inflence of serotyping of Aeromonas hydrophila on yolk immunoglobulin(IgY),Aer toxin and ECPase of Aeromonas hydrophila were extracted and identified followed by being immuned to layer chickens.IgY in egg yolks was separated with octoic acid-ammonium sulfate.Indirect ELISA was used to assay the titer of IgY and the cross-reactivity to different Aer toxins and ECPases from different sources.The titer of the IgY to Aer toxins and ECPase peaked at 1∶213 and 1∶212,respectively.Strong cross-reactions were showed between IgY and different Aer toxins or ECPases from other sources. |
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| Keywords: | Aeromonas hydrophila Aer toxin ECPase IgY cross-reactivity |
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