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| 茄子黄萎病生防内生细菌29-12发酵条件的初步研究 | |
| 引用本文： | 孙义,居正英,林玲,杨启银,周益军.茄子黄萎病生防内生细菌29-12发酵条件的初步研究[J].江苏农业学报,2008,24(4). |
| 作者姓名： | 孙义 居正英 林玲 杨启银 周益军 |
| 作者单位： | 1. 南京师范大学生命科学学院,江苏,南京,210046;江苏省农业科学院植物保护研究所,江苏,南京,210014 2. 江苏省农业科学院植物保护研究所,江苏,南京,210014 3. 南京师范大学生命科学学院,江苏,南京,210046 |
| 基金项目： | 国家高技术研究发展计划(863计划)，江苏省自然科学基金，国家科技支撑计划，公益性行业(农业)科研专项，江苏省农业科学院资助项目 |
| 摘 要： | 初步探讨茄子黄萎病生防内生性枯草芽孢杆菌29-12的发酵条件,通过培养条件优化及正交试验得出最佳培养基配方为:玉米粉15 g/L、甘薯淀粉5 g/L、豆粕粉15 g/L、蛋白胨8 g/L、MgSO4 0.01%、K2HPO4 0.05%.最佳培养条件为:起始pH值7.5,250 ml三角瓶装液量100 ml,摇瓶速度120 r/min,接种量10%,温度30 ℃.在此条件下培养29-12菌株 84 h,抑菌圈大小为2.12 cm,1 ml含菌量为1.58×109 , 芽孢得率为100%. |
| 关 键 词： | 枯草芽孢杆菌 正交设计 发酵条件 |
Fermentation Conditions of Endophytic Bacterium Strain 29-12 Biocontrolling Eggplant Verticillium dahliae |
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| SUN Yi,JU Zheng-ying,LIN Ling,YANG Qi-yin,ZHOU Yi-jun.Fermentation Conditions of Endophytic Bacterium Strain 29-12 Biocontrolling Eggplant Verticillium dahliae[J].Jiangsu Journal of Agricultural Sciences,2008,24(4). | |
| Authors: | SUN Yi JU Zheng-ying LIN Ling YANG Qi-yin ZHOU Yi-jun |
| Institution: | SUN Yi1,2,JU Zheng-ying2,LIN Ling2,YANG Qi-yin1,ZHOU Yi-jun2(1.College of Life Science,Nanjing Normal University,Nanjing 210046,China,2.Institute of Plant Protection,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China) |
| Abstract: | To work out the fermentation conditions of endophytic bacterium strain 29-12 of Bacillus subtilis antagonistic to Verticillium dahliae from eggplant,culture condition was optimized and orthogonal tests were designed.The optimal culture medium composition was showed as corn meal 15 g/L,poi starch 5 g/L,soybean meal 15 g/L,peptone 8 g/L,MgSO4 0.01%,and K2HPO4 0.05%.When the strain was cultured in above medium with the following conditions as initial pH 7.5,inoculation level 10%,a 120 r/min shaking revolution ... |
| Keywords: | Bacillus subtills orthogonal design fermentation condition |
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