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海蓬子DnaJ-like基因片段的表达和生物信息学分析
引用本文:张大栋,周春霖,任丽娟,王茂文,汤日圣,马鸿翔.海蓬子DnaJ-like基因片段的表达和生物信息学分析[J].江苏农业学报,2006,22(3):222-224.
作者姓名:张大栋  周春霖  任丽娟  王茂文  汤日圣  马鸿翔
作者单位:1. 江苏省农业科学院农业生物遗传生理研究所,江苏,南京,210014
2. 江苏省沿海地区农业科学研究所,江苏,盐城,224002
基金项目:江苏省自然科学基金;江苏省农业科学院资助项目
摘    要:从盐生植物海蓬子的抑制消减杂交文库中克隆了DnaJ-like基因的长609 bp的DNA序列,编码1个202 aa的开放阅读框,Northern B loting显示该基因在检测子(200 mmol/L NaC l)中的表达显著增强。生物信息学分析表明,在蛋白质水平上,该片段与拟南芥和水稻的同源性分别为6 e-68和1 e-58;在核酸水平上,该片段与拟南芥的同源性为3 e-24。保守区分析显示,该片段含有DnaJ-like基因完整的J功能域(J-Dom ain,66 aa),功能域在蛋白质水平与GenBank两种相关类型的J功能域的同源性分别为1 e-11和9 e-15。这为该基因的克隆及研究其在植物逆境胁迫中的作用奠定了基础。

关 键 词:DnaJ-like基因  表达  生物信息学分析  海蓬子
文章编号:1000-4440(2006)03-0222-03
收稿时间:2005-12-05
修稿时间:2005-12-05

Expression and Bioinformatics Analysis of DnaJ-like Gene Sequence from Salicornia bigelovii Torr.
ZHANG Da-dong,ZHOU Chun-lin,REN Li-juan,WANG Mao-wen,TANG Ri-sheng,MA Hong-xiang.Expression and Bioinformatics Analysis of DnaJ-like Gene Sequence from Salicornia bigelovii Torr.[J].Jiangsu Journal of Agricultural Sciences,2006,22(3):222-224.
Authors:ZHANG Da-dong  ZHOU Chun-lin  REN Li-juan  WANG Mao-wen  TANG Ri-sheng  MA Hong-xiang
Institution:1. Institute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricuhural Sciences, Nanjing 210014, China; 2. Institute of Agricultural Sciences in the Coastal District of Jiangsu, Yancheng 224002, China
Abstract:A 609 bp DNA sequence of DnaJ-like gene was successfully cloned from halophyte,Salicornia Bigelovii Torr.,during early stage of salt stress based on suppression subtractive hybridization(SSH) and comfirmed by BLAST x/n analysis. The result of Northern bloting indicated that the represented gene was significantly upregulatedly expressed under 200 mmol/L NaCl treatment compared to non-NaCl treatment.Bioinformatics analysis showed that the sequence of DnaJ-like gene in halophyte has high E-values,6e-68 and 1e-58,when compared with Arabidopsis thaliana and Oryza sativa at amino acid level(Blastx) and the E-value reached 3e-24 when compared with Arabidopsis thaliana DnaJ-like cDNA sequcnce at nucleotide level(Blastn).Conserved domain analysis made it clear that an integrated J-domain was involved in the sequence.J-domain has E value of 1e-11 and 9e-15 compared with two types J-domains in GenBank CDD database.All these have widely suggested that it will be expectable to clone the full length cDNA of DnaJ-like gene from the inherent high salt-tolerant species.
Keywords:DnaJ-like gene  expression  bioinformatics analysis  haloqhyte  
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