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波尔山羊活体采卵与卵母细胞体外受精技术
引用本文:王公金,季尚娟,徐晓波,赵伟,陈舒兵.波尔山羊活体采卵与卵母细胞体外受精技术[J].江苏农业学报,2004,20(3):154-158.
作者姓名:王公金  季尚娟  徐晓波  赵伟  陈舒兵
作者单位:1. 江苏省农业科学院畜牧研究所,江苏,南京,210014
2. 南京农业大学动物科学院,江苏,南京,210095
基金项目:江苏省自然科学基金项目(BK2002125);南京市科技局科研项目(20005008)
摘    要:对10只不孕不育波尔母山羊采用超数排卵、活体采卵、卵母细胞体外成熟培养以及体外受精等技术方法,探讨其是否能正常繁育后代。试验中6只供体经超数排卵处理,在发情前利用特制的采卵装置进行活体采卵,共采集到30枚形态学正常的卵母细胞。经过体外成熟培养,有21枚成熟卵母细胞进行了体外受精处理和培养,获得16枚2 细胞胚胎,移植到4只同期发情的当地山羊受体输卵管内,经妊娠检验,有两只受体受孕,其中1只受体产出正常羔羊2只。该技术经进一步改进完善后可望作为不孕不育波尔山羊繁殖后代的辅助生育技术。

关 键 词:波尔山羊  活体采卵  卵母细胞  体外受精
文章编号:1000-4440(2004)03-0154-05
修稿时间:2004年2月5日

Ovum Pick up and Fertilization in vitro of Follicular Oocytes for Boer Goats
WANG Gong-jin,JI Shang-juan,XU Xiao-bo,ZHAO Wei,CHEN Shu-bing.Ovum Pick up and Fertilization in vitro of Follicular Oocytes for Boer Goats[J].Jiangsu Journal of Agricultural Sciences,2004,20(3):154-158.
Authors:WANG Gong-jin  JI Shang-juan  XU Xiao-bo  ZHAO Wei  CHEN Shu-bing
Institution:WANG Gong-jin~1,JI Shang-juan~2,XU Xiao-bo~1,ZHAO Wei~1,CHEN Shu-bing~1
Abstract:Ten sterile female boer goat donors were tested in this research,which were approached to breed normal offsprings by the techniques of superovulation,ovum pick up,maturation culture in vitro and fertilization in vitro of the maturated oocytes etc.In this experiment 6 donors treated by superovulation were operated to collected follicular oocytes by a special ovum pick up equipment before their oestrus.Thirty oocytes were recovered from ten ovaries of several donors.Twenty-one oocytes after maturation in vitro were fertilized in vitro and cultured in vitro. Sixteen 2-cell embryos that survived were transfered into oviducts of four local female goats which had been treated by synchronization of oestrus.Two of the 4 recipients were pregnant during three months.Two normal offsprings were borne from one of the pregnant recipients.This technique was expected to improve further as an alternative reproductive method for the sterile female boer goats.
Keywords:Boer goat  ovum pick up  follicular oocyte  fertilization in vitro
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