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转CpTI基因不结球白菜的抗虫性表现
引用本文:佘建明,何晓兰,蔡小宁,苏小俊,袁希汉,李彬.转CpTI基因不结球白菜的抗虫性表现[J].江苏农业学报,2004,20(4):230-232.
作者姓名:佘建明  何晓兰  蔡小宁  苏小俊  袁希汉  李彬
作者单位:1. 江苏省农业科学院农业生物遗传生理研究所,江苏,南京,210014
2. 江苏省农业科学院蔬菜研究所,江苏,南京,210014
基金项目:江苏省科技厅农业高新技术项目(BG2002308)
摘    要:通过卡那霉素筛选、目的基因分子检测和生物学抗虫性鉴定 ,从转豇豆胰蛋白酶抑制剂基因不结球白菜遗传工程植株自交后代中选育出抗虫性可遗传的纯合材料。用室内人工饲喂方法 ,以鳞翅目小菜蛾、斜纹夜蛾幼虫为靶害虫 ,对转基因植株进行抗虫性鉴定。试验结果显示 ,转CpTI基因纯合材料对 1~ 2龄小菜蛾和斜纹夜蛾幼虫具有抗性 ,但不同株系间和同一株系内植株间的抗虫性程度差异较大

关 键 词:不结球白菜  豇豆胰蛋白酶抑制剂基因  抗虫性  小菜蛾  斜纹夜蛾
文章编号:1000-4440(2004)04-0230-03
修稿时间:2004年2月14日

Expression of Insect Resistance in CpTI Transgenic Plants of Non-heading Chinese Cabbage
SHE Jian-ming,HE Xiao-lan,CAI Xiao-ning,SU Xiao-jun,YUAN Xi-han,LI Bin.Expression of Insect Resistance in CpTI Transgenic Plants of Non-heading Chinese Cabbage[J].Jiangsu Journal of Agricultural Sciences,2004,20(4):230-232.
Authors:SHE Jian-ming  HE Xiao-lan  CAI Xiao-ning  SU Xiao-jun  YUAN Xi-han  LI Bin
Institution:SHE Jian-ming~1,HE Xiao-lan~1,CAI Xiao-ning~1,SU Xiao-jun~2,YUAN Xi-han~2,LI Bin~2
Abstract:Through kanamycin screening,PCR-Southern blot analysis and insect resistance identification,the homozygous material with insect resistance was selected from the transgenic plant progenies of non-heading Chinese cabbage.The larvae of Plutella xylostella (Linnaeus)and Prodenia litura Fabricius as target insects,the insect resistance bioassay had been made for CpTI transgenic plants by the artificial feed.The test showed that the transgenic homozygous material had insect resistance to the first or second instar larvae of Plutella xylostella (Linnaeus) and Prodenia litura Fabricius.The degree of insect resistance was different obviously in lines or plant of the same line.
Keywords:non-heading Chinese cabbage  cowpea trypsin inhibitor(CpTI) gene  insect resistance  Plutella xylostella(Linnaeus)  Prodenia litura Fabricius
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