夏蜡梅腋芽微繁殖及叶片不定芽再生

夏蜡梅(Sinocalycanthus chinensis)是中国特有的一种落叶观赏花木,为国家二级保护珍稀濒危植物.以夏蜡梅当年生枝条的茎段为供试材料,进行腋芽微繁殖和叶片不定芽再生的研究.结果表明:茎段在附加2.00mg/L ZT和0.05 ms/L IBA的WPM培养基上快繁,萌发迅速、芽体粗壮、叶色淡绿,月繁殖系数超过10.0,可以连续继代10次以上;以无菌苗的叶片为外植体在附加2.0 me/L TDZ、0.1 me/L IBA和1.0 me/L KT的WPM培养基上,不定芽再生频率达40%.在3/5MS大量元素+WPM微量元素+WPM有机成分+0.20 mg/L IBA+0.05%活性炭的生根培养基上,试管苗的根系和地上部发育良好,盆钵移栽成活率达100%.该体系的建立为夏蜡梅工厂化育苗以及运用生物技术手段对夏蜡梅进行改良奠定了基础. ms/L IBA的WPM培养基上快繁,萌发迅速、芽体粗壮、叶色淡绿,月繁殖系数超过10.0,可以连续继代10次以上;以无菌苗的叶片为外植体在附加2.0 me/L TDZ、0.1 me/L IBA和1.0 me/L KT的WPM培养基上,不定芽再生频率达40%.在3/ MS大量元素+WPM微量元素+WPM有机成分+0.20 mg/L IBA+0.05%活性炭的生根培养基上,试管苗的根系和地上部发育良好,盆钵移栽成活率达100%.该体系的建立为

Micropropagation of Axillary Bud and Adventitious Shoot Regeneration from Chinese Sweetshrubs (Sinocalycanthus chinensis) Leaf Explants

Chinese sweetshrubs (Sinocalycanthus chinensis), an endangered species in China and therefore entered in the list of the class Ⅱ protected plant, is one of endemic deciduous ornamental tree. In this experiment, a stem-based micropropagation system for this species was established. The results showed that a micropropagation rate of axillary bud was high up to 10. 0 per month, and might be 10 continuous subcultures on WPM medium supplemented with 2. 00 mg/LZT and 0. 05 mg/L IBA. Adventitious shoots were regenerated from leaf explants which derived from micropropagated shoots at second continuous culture stage. The highest regeneration rate (40%) and clustered adventitious buds were obtained on WPM basal medium supplemented with 2.0 mg/L TDZ, 0. 1 mg/L IBA and 1.0 mg/L KT. Plantlets from axillary and ad-ventitious buds rooted on the root medium with 0. 2 mg/L IBA and then successfully transplanted into the soil. The micro-propagation and regeneration systems for the species layed the foundation for its seedlings commercialization and provided essential skills for its biotechnology-based improvement.