| 花粉管介导的转抗冻蛋白基因(AFP)水稻 |
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| 引用本文： | 赵凌,王才林,张亚东,朱镇,杨杰,黄骏麒,龚蓁蓁.花粉管介导的转抗冻蛋白基因(AFP)水稻[J].江苏农业学报,2006,22(4):315-317. |
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| 作者姓名： | 赵凌 王才林 张亚东 朱镇 杨杰 黄骏麒 龚蓁蓁 |
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| 作者单位： | 1. 江苏省农业科学院粮食作物研究所,江苏省优质水稻工程技术研究中心,江苏,南京,210014
2. 中国科学院上海生物化学研究所,上海,200031 |
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| 基金项目： | 农业结构调整技术专项基金;江苏省高技术研究发展计划项目 |
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| 摘 要： | 低温和冷害在世界范围内广泛发生,对水稻的产量和品质造成严重影响。创造和培育具有强耐低温特性的水稻品种,对提高水稻产量具有重要的意义。本研究将从胡萝卜中克隆的抗冻蛋白基因(AFP)构建到植物表达载体pB I121(pB I121∶AFP)后,利用花粉管通道法将其转入籼稻品种NR158,获得了转基因后代。对T3代和T4代转基因植株的苗期耐冷性鉴定和PCR鉴定结果表明,部分转基因植株后代已经整合并且AFP基因得到了表达,获得了不同程度的耐低温性。
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| 关 键 词： | 水稻(Oryza sativa L.) 花粉管通道法 耐低温性 抗冻蛋白基因(AFP) 转基因植株 |
| 文章编号： | 1000-4440（2006）04-0315-03 |
| 收稿时间： | 2006-07-10 |
| 修稿时间： | 2006-07-10 |
Transformation of Antifreeze Protein Gene (AFP) from Carrot (Daucus carota) into Rice (Oryza sativa L.)by Pollen-tube Pathway |
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| ZHAO Ling,WANG Cai-lin,ZHANG Ya-dong,ZHU Zhen,YANG Jie,HUANG Jun-qi,GONG Zhen-zhen.Transformation of Antifreeze Protein Gene (AFP) from Carrot (Daucus carota) into Rice (Oryza sativa L.)by Pollen-tube Pathway[J].Jiangsu Journal of Agricultural Sciences,2006,22(4):315-317. |
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| Authors: | ZHAO Ling WANG Cai-lin ZHANG Ya-dong ZHU Zhen YANG Jie HUANG Jun-qi GONG Zhen-zhen |
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| Abstract: | Low temperature and chilling injury are natural disasters in the world,resulting in reduction of rice output and rice quality.Creation of new rice resources and breeding of new rice varieties with high tolerance to low temperature and chilling injury are critical to increase rice production.The antifreeze protein gene(AFP) constructed to expression vector PBI 121 was transferred into indica rice NR158 by pollentube pathway method in this study to improve its sensitivity to low temperature. Some transgenic rice plants were obtained.PCR analyses and cold tolerance appraisal for T_3-and T_4-generation transgenic rice plants in seedling stage proved that AFP gene were expressed in some transgenic plants,leading to the acguisition of low temperature tolerance. |
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| Keywords: | rice(Oryza sativa L ) pollen-tube pathway method low temperature tolerance antifreeze protein gene(AFP) transgenic plant |
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