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| 重离子束12C+6诱变柠檬酸产生菌的培养基优化 | |
| 引用本文： | 胡伟,陈积红,张珍,刘敬,王曙阳,李文建,周林.重离子束12C+6诱变柠檬酸产生菌的培养基优化[J].湖南农业科学,2012(7):104-106. |
| 作者姓名： | 胡伟 陈积红 张珍 刘敬 王曙阳 李文建 周林 |
| 作者单位： | 1. 中国科学院近代物理研究所,甘肃兰州730000;甘肃农业大学食品科学与工程学院,甘肃兰州730030 2. 中国科学院近代物理研究所,甘肃兰州,730000 3. 甘肃农业大学食品科学与工程学院,甘肃兰州,730030 |
| 基金项目： | 中国科学院科技支甘项目 |
| 摘 要： | 采用了三因素三水平正交试验研究了培养基主要成分淀粉、豆饼粉及麸皮对柠檬酸生产菌黑曲霉H4002发酵结果的影响,同时采用梯度试验考察了丙酮添加对最后发酵酸度的影响。试验结果表明相对最优的黑曲霉培养条件为:36.9℃、300 r/min、发酵时间75 h,淀粉、豆饼粉及麸皮含量分别为18%、0.45%和0.45%时,柠檬酸酸度最高,为100.8 g/L。 |
| 关 键 词： | 培养基 正交试验 丙酮 |
Optimization of Culture Medium for Inducing Citric Acid Producing Strain by Heavy Ion Beam 12C+6 |
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| HU Wei , CHEN Ji-hong , ZHANG zhen , LIU Jing , WANG Shu-yang , LI Wen-jian , ZHOU Lin.Optimization of Culture Medium for Inducing Citric Acid Producing Strain by Heavy Ion Beam 12C+6[J].Hunan Agricultural Sciences,2012(7):104-106. | |
| Authors: | HU Wei CHEN Ji-hong ZHANG zhen LIU Jing WANG Shu-yang LI Wen-jian ZHOU Lin |
| Institution: | 1,2(1.Institute of Modern Physics,Chinese Academy of Sciences,Lanzhou 730000,PRC; 2.College of Food Science and Engineering,Gansu Agricultural University,Lanzhou 730070,PRC) |
| Abstract: | The effects of three main components of culture medium(starch,bean cake powder and wheat bran) on fermentation results of citric acid producing strain Aspergillus niger H4002 were studied by orthogonal experiment with three factors and three levels.Meanwhile,the effect of adding acetone on final fermentation acidity was studied by gradient test.The results indicated that the optimal conditions for culture of Aspergillus niger were 36.9℃,300 r/min,75 h of fermentation time.When contents of starch,bean cake powder and wheat bran were 18%,0.45% and 0.45%,respectively,the acidity of citric acid was the highest(100.8 g/L). |
| Keywords: | culture medium orthogonal experiment acetone |
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