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| 烟用香精香料对人支气管上皮细胞的毒性研究 | |
| 引用本文： | 谢慧玲,胡旺顺,钟波,张发明,李映相,李发平,苏仕开,刘姜瑾.烟用香精香料对人支气管上皮细胞的毒性研究[J].湖南农业科学,2009(1). |
| 作者姓名： | 谢慧玲 胡旺顺 钟波 张发明 李映相 李发平 苏仕开 刘姜瑾 |
| 作者单位： | 1. 云南省保山市烟草公司施甸县分公司,云南施甸,678200 2. 湖南中烟工业有限责任公司技术中心,湖南长沙,410014 3. 云南省保山市烟草公司,云南保山,678000 |
| 摘 要： | 为了探讨烟用香精香料对人支气管上皮细胞的毒性作用,并为其安全性评价提供毒理学依据,将永生化的人支气管上皮细胞(BEP2D细胞)分为纯卷烟组和卷烟加香加料组、卷烟加香精和卷烟加香料组、卷烟加不同香精组、卷烟加不同香料组,用MTT法测定细胞存活率,用分子探针氢化乙锭(HE)的荧光产物溴乙锭(EB)相对定量细胞内活性氧(ROS)水平.结果表明加香加料致卷烟烟气冷凝物(CSC)的细胞存活率降低,细胞内ROS水平增高,对细胞的氧化损伤大;与香料比较,香精的细胞存活率低,对细胞的氧化损伤大;1#香精的细胞存活率较高,但对细胞的氧化损伤较大;1#香料的细胞存活率较低,但对细胞的氧化损伤较小,表现在细胞内ROS水平较低.由此町知CSC对BEP2D细胞有毒性,烟用香精香料的添加增加了烟气对BEP2D细胞的毒性作用,而在香精和香料中,香精对细胞毒性较大. |
| 关 键 词： | 烟用香精香料 人支气管上皮细胞 细胞存活率 活性氧 卷烟烟气冷凝物 细胞毒性 |
Study on Cytotoxicity of Tobacco Essence and Flavor on BEP2D Cells |
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| XIE Hui-ling,HU Wang-shun,ZHONG Bo,ZHANG Fa-ming,LI Ying-xiang,LI Fa-ping,SU Shi-kai,LIU Jiang-jin.Study on Cytotoxicity of Tobacco Essence and Flavor on BEP2D Cells[J].Hunan Agricultural Sciences,2009(1). | |
| Authors: | XIE Hui-ling HU Wang-shun ZHONG Bo ZHANG Fa-ming LI Ying-xiang LI Fa-ping SU Shi-kai LIU Jiang-jin |
| Institution: | 1. Tobacco Company of Shidian;Shidian 678200;PRC;2. Technology Center of China Tobacco Hunan Industrial Corporation;Changsha 410014;3. Tobacco Company of Baoshan;Baoshan 678000;PRC |
| Abstract: | The toxic effects of tobacco essence and flavor on human bronchial epithelial cells were studied to provide tox-icological basis for the safety evaluation. Exponentially growing immortalized human bronchial epithelial (BEP2D) cells were used. The experiments were carried out contained of (1) the pure tobacco group and tobacco essence and flavor group, (2) tobacco essence group and tobacco flavor group, (3) different tobacco essence group, (4) different tobacco flavor group. The cell survival rates were meas... |
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