| 好氧反硝化细菌LKX-1菌株培养基与发酵条件的优化 |
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| 引用本文： | 赵健宇,刘立立,兰时乐,杨友才.好氧反硝化细菌LKX-1菌株培养基与发酵条件的优化[J].湖南农业科学,2017(4). |
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| 作者姓名： | 赵健宇 刘立立 兰时乐 杨友才 |
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| 作者单位： | 湖南农业大学生物科学技术学院,湖南 长沙,410128 |
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| 基金项目： | 湖南省农业委员会2016年湖南重金属污染耕地修复及农作物种植结构调整试点资金专项，湖南省环保科研项目 |
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| 摘 要： | 采用单因素试验法研究了蒙氏假单胞菌LKX-1菌株的发酵培养基组成和发酵条件,并采用正交试验法对发酵条件进行了优化。结果表明:适宜的发酵培养基配方为:麦芽糖2.0%,酵母粉2.0%,KH_2PO_4 0.15%,MgSO_4·7H_2O 0.10%;最适发酵条件为:发酵温度30℃,接种量1.0%,初始pH值7.0,装液量100 m L/瓶(三角瓶容量250 m L),发酵时间24 h;在此条件下,发酵液中活菌数可达4.5×10~(14)CFU/m L。
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| 关 键 词： | 好氧反硝化细菌 培养基 发酵条件 优化 |
Studies on the Culture Medium and Fermentation Conditions of the Aerobic Denitrifier LKX-1 |
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| ZHAO Jian-yu,LIU Li-li,LAN Shi-le,YANG You-cai.Studies on the Culture Medium and Fermentation Conditions of the Aerobic Denitrifier LKX-1[J].Hunan Agricultural Sciences,2017(4). |
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| Authors: | ZHAO Jian-yu LIU Li-li LAN Shi-le YANG You-cai |
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| Abstract: | The composition of fermentation medium and fermentation conditions were studied by using single-factor test, and the fermentation conditions were optimized by using orthogonal experiment. The results showed that the optimal fermentation medium were as follows: maltose 2%, yeast powder 2%, KH2PO40.15%, MgSO4·7H2O 0.10%, and the optimal fermentation conditions were as follows:fermentation temperature 30℃ , inoculation 1%, the initial pH 7.0, the liquid volume 100 mL/ bottle, fermentation time 24 h. Under these conditions, the viable count reached 4.5×1014 CFU/mL. |
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| Keywords: | aerobic denitrifier culture medium fermentation conditions optimization |
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