植物转基因抗性策略研究进展

通过转基因技术使植物获得抗病性是控制植物病毒感染的一种重要技术途径。RNA干扰技术的出现为植物转基因抗性策略的研究提供了新思路。植物转基因抗性产生方法主要是通过表达不同的病毒蛋白(外壳蛋白、复制蛋白、运动蛋白及其他病毒蛋白)、RNAs(反义RNA、卫星RNA、缺陷干扰RNA、发夹RNA及人工微小RNA)、非病毒基因(核酸酶、抗病毒蛋白抑制子及植物体)、宿主来源的抗性基因(显性抗性基因和隐性抗性基因)及各种宿主防御反应因子等。介绍了以上几种抗性策略并分析了目前尚存在的问题,以期为植物转基因抗性策略研究的学者提供参考。

Research Advances on Transgenic Resistance Strategies in Plants

Transgenic resistance to plant virus was an important technology for control of plant virus infection. The development of RNA interference techniques lead a new way to the research on transgenic resistance strategies in plants. The approaches used to confer resistance in plants were based on the expression of various viral proteins (capsid proteins, replicase proteins, movement proteins and other viral proteins), RNAs (antisense RNAs, satellite RNAs, defective interfering RNAs, hairpin RNAs, and artiifcial microRNAs), nonviral genes (nucleases, antiviral inhibitors, and plantibodies), host-derived resistance genes (dominant resistance genes and recessive resistance genes) and various factors involved in host defense responses. This paper chielfy introduced the strategies used to confer resistance in plants and analyzed the problem of this area, which in order to provide the reference for the researchers.