| 东方百合花器离体培养和快速繁殖研究 |
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| 引用本文： | 柯昉,陈华,黄宇翔,李章汀.东方百合花器离体培养和快速繁殖研究[J].仲恺农业技术学院学报,2005,18(1):14-17. |
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| 作者姓名： | 柯昉 陈华 黄宇翔 李章汀 |
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| 作者单位： | 福建省农业科学院,地热农业利用研究所,福建,福州,350003 |
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| 摘 要： | 以东方百合(Lilium tenuifolium oriental hybrid)索邦品种的花瓣、花丝、花托为外植体,就不同激素组合对诱导愈伤组织和不定芽的影响进行了研究.结果表明,索邦百合花瓣、花丝、花托形成愈伤组织的能力有差异,其能力大小依次为花托＞花丝＞花瓣;愈伤组织诱导的最佳培养基为MS 2,4-D 1.0 mg/L BA 0.5 mg/L,芽最佳分化培养基为MS BA 1.0 mg/L NAA 0.1 mg/L,芽最佳增殖培养基为MS KT 0.5 mg/L NAA 0.1 mg/L,生根最佳培养基为1/2 MS NAA 0.2mg/L.
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| 关 键 词： | 东方百合(Lilium tenuifolium oriental hybrid) 花器培养 快速繁殖 |
| 文章编号： | 1006-0774(2005)01-0014-04 |
| 修稿时间： | 2004年8月30日 |
Study on floral organ culture and rapid propagation of oriental hybrid lily |
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| KE Fang,CHEN Hua,HUANG Yu-xiang,LI Zhang-ting.Study on floral organ culture and rapid propagation of oriental hybrid lily[J].Journal of Zhongkai Agrotechnical College,2005,18(1):14-17. |
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| Authors: | KE Fang CHEN Hua HUANG Yu-xiang LI Zhang-ting |
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| Abstract: | The floral organ culture of Oriental Lily Sorbonne was studied by using its petals,filaments and receptacles as explants respectively. The best explant was receptacle. The best medium for callus formation was MS+2,4-D 1.0 mg/L+BA 0.5 mg/L. The best medium for bud initiation was MS+BA1.0 mg/L+NAA0.1 mg/L. The best medium for the propagation of cluster shoots was MS+KT0.5 mg/L+NAA0.1 mg/L. The best medium for rooting of seedlings was 1/2MS +NAA 0.2 mg/L. |
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| Keywords: | L tenuifoliumoriental hybrid floral organ culture rapid propagation |
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