| 猪链球菌及其血清型的PCR检测 |
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| 引用本文： | 臧莹安,胡波,卢頔,宋帅,李淼,李春玲.猪链球菌及其血清型的PCR检测[J].仲恺农业技术学院学报,2011,24(3):9-11,19. |
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| 作者姓名： | 臧莹安 胡波 卢頔 宋帅 李淼 李春玲 |
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| 作者单位： | 1. 仲恺农业工程学院生命科学学院,广东广州,510225
2. 广东省农业科学院兽医研究所,广东广州,510640 |
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| 基金项目： | 国家自然科学基金联合资助项目，国家公益性行业(农业)科研专项 |
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| 摘 要： | 从广东省部分地区猪场采集疑似猪链球菌病病猪的扁桃体棉拭病料202份，用PCR方法检测棉拭样本培养物中猪链球菌（Streptococcussuis）及其主要致病血清型（1、2、7和9型）．检出阳性样品147份，检出率为72．8％．其中检出S．suis血清2型21份，检出率为12．9％；检出血清9型6份，检出率为3．0％；未检出血清7型和1型．此外，不同地区和不同发育阶段的猪，S．suis检出率和各血清型检出情况也存在较大差异．从而证实广东地区猪群中广泛存在S．suis呈地域性流行，并且是多种血清型共存．
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| 关 键 词： | 猪链球菌（Streptococcus suis） PCR 血清型 检出率 |
Detection of Streptococcus suis and their serotypes by PCR |
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| ZANG Ying-an,HU Bo,LU Di,SONG Shuai,LI Miao,LI Chun-ling.Detection of Streptococcus suis and their serotypes by PCR[J].Journal of Zhongkai Agrotechnical College,2011,24(3):9-11,19. |
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| Authors: | ZANG Ying-an HU Bo LU Di SONG Shuai LI Miao LI Chun-ling |
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| Institution: | ZANG Ying-an1,HU Bo1,LU Di1,SONG Shuai2,LI Miao2,LI Chun-ling2(1.College of Life Sciences,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China,2.Institute of Veterinary Science,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China) |
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| Abstract: | 202 swine tonsilla swabs collected from some areas in Guangdong province were detected for Streptococcus suis and their serotypes（ I ,2,7,9 ）, using the PCR methods. 147 S. suis strains were detected and the detection rate was 72. 8%. Among of them, 21 samples were S. suis serotype 2, 6 samples were serotype 9, serotype 1 and 7 strains were not detected. The detection rate of S. suis and the Serotypes were varied significantly among different areas and growth stages of pigs. The results showed that S. suis were existed widely in pigs, and multiple serotypes of S. suis seemed to be enzootic prevalence in Guangdong. |
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| Keywords: | Streptococcus suis PCR serotypes detection rate |
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