| 广东省桔小实蝇种群感染Wolbachia的分子检测 |
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| 引用本文： | 李红梅,陈泽炳,林进添.广东省桔小实蝇种群感染Wolbachia的分子检测[J].仲恺农业技术学院学报,2010,23(1). |
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| 作者姓名： | 李红梅 陈泽炳 林进添 |
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| 作者单位： | 1. 仲恺农业工程学院,生命科学学院,广东,广州,510225
2. 仲恺农业工程学院,农学院,广东,广州,510225 |
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| 基金项目： | 国家自然科学基金，广东省自然科学基金 |
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| 摘 要： | 沃尔巴克氏体(Wolbachia)是一类广泛存在于节肢动物生殖器官内的共生细菌.利用Wolbachia表面蛋白wsp基因的1对通用引物81F和691R,对广东省桔小实蝇(Bactrocera dorsalis)地理种群感染Wolbachia状况进行PCR检测.结果表明,桔小实蝇珠海地理种群有1个个体感染Wolbachia,其他种群均未见感染;广东省桔小实蝇种群感染Wolbachia的概率为0.4%.
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| 关 键 词： | 桔小实蝇(Bactrocera dorsalis) wsp基因 沃尔巴克氏体(Wolbachia) 分子检测 |
Molecular detection on Wolbachia infecting Bactrocera dorsalis populations of Guangdong |
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| LI Hong-mei,CHEN Ze-bing,LIN Jin-tian.Molecular detection on Wolbachia infecting Bactrocera dorsalis populations of Guangdong[J].Journal of Zhongkai Agrotechnical College,2010,23(1). |
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| Authors: | LI Hong-mei CHEN Ze-bing LIN Jin-tian |
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| Institution: | LI Hong-mei1,CHEN Ze-bing1,LIN Jin-tian2(1.College of Life Sciences,Zhongkai University of Agriculture , Engineering,Guangzhou 510225,China,2.College of Agronomy,China) |
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| Abstract: | Polymerase chain reaction(PCR) with general Wolbachia surface protein(wsp) primers 81F and 691R was used to detect the presence of Wolbachia in Bactrocera dorsalis geographical populations of Guangdong.The results showed that only one individual of B.dorsalis in Zhuhai geographic population was infected by Wolbachia.However,the other geographic populations were not infected,and the infection rate of Wolbachia in B.dorsalis populations from Guangdong was 0.4%. |
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| Keywords: | Bactrocera dorsalis wsp gene Wolbachia molecular detection |
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