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| 菜豆细菌性萎蔫病菌16S rDNA基因克隆及TaqMan探针实时荧光PCR检测 | |
| 引用本文： | 漆艳香,朱水芳,肖启明.菜豆细菌性萎蔫病菌16S rDNA基因克隆及TaqMan探针实时荧光PCR检测[J].仲恺农业技术学院学报,2004,17(4):10-17. |
| 作者姓名： | 漆艳香 朱水芳 肖启明 |
| 作者单位： | 1. 中国热带农业科学院,环境与植物保护研究所,海南,儋州,571737 2. 国家质检总局动植物检疫实验所,北京100029 3. 湖南农业大学,农学院,湖南,长沙,410128 |
| 基金项目： | 国家质检总局植物病原检测专项基金 |
| 摘 要： | 将菜豆细菌性萎蔫病菌(Curtobacterium flaccumfaciens pv. flaccumfaciens)16S rDNA基因进行克隆测序,并依据此序列设计菜豆细菌性萎蔫病菌的实时荧光PCR引物和特异性探针,对所收集的样品进行了检测. 结果表明,该检测方法具有快速、特异、灵敏、重复性好等优点,只有菜豆细菌性萎蔫病菌产生荧光,其他细菌没有荧光产生. 检测的灵敏度为105 CFU/mL的细菌悬浮液,相对灵敏度为106 CFU/mL. |
| 关 键 词： | 菜豆细菌性萎蔫病菌(Curtobacterium flaccumfaciens pv. flaccumfaciens) 实时荧光PCR 检测 |
| 文章编号： | 1006-0774(2004)04-0010-08 |
| 修稿时间： | 2004年8月23日 |
Curtobacterium flaccumfaciens pv.flaccumfaciens detected by real-time fluorescent PCR |
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| QI Yan-xiang,ZHU Shui-fang,XIAO Qi-ming.Curtobacterium flaccumfaciens pv.flaccumfaciens detected by real-time fluorescent PCR[J].Journal of Zhongkai Agrotechnical College,2004,17(4):10-17. | |
| Authors: | QI Yan-xiang ZHU Shui-fang XIAO Qi-ming |
| Institution: | QI Yan-xiang~1,ZHU Shui-fang~2,XIAO Qi-ming~3 |
| Abstract: | The 16S rDNA gene of Curtobacterium flaccumfaciens pv.flaccumfaciens was cloned and sequenced, and it was detected by real-time fluorescent PCR. A subgroup-specific probe was designed based on its sequence, which was used to detect 10 bacteria. The results showed that no signal could be detected from the other bacteria except Curtobacterium flaccumfaciens pv. flaccumfaciens with the relative sensitivity was 10~6 CFU/mL and that of the suspension of pure culture was 10~5 CFU/mL. The described method has the advantage of high speed, sensitivity, specificity and stable reproduction. |
| Keywords: | Curtobacterium flaccumfaciens pv flaccumfaciens real-time fluorescent PCR detection |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |