| 枯草芽孢杆菌Snb2原生质体制备和再生研究 |
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| 引用本文： | 王媛媛,段玉玺,陈立杰,朱晓峰.枯草芽孢杆菌Snb2原生质体制备和再生研究[J].沈阳农业大学学报,2011,42(1):110-113. |
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| 作者姓名： | 王媛媛 段玉玺 陈立杰 朱晓峰 |
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| 作者单位： | 1. 沈阳农业大学生物科学技术学院,沈阳,110161;沈阳农业大学植物保护学院,沈阳,110161
2. 沈阳农业大学植物保护学院,沈阳,110161 |
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| 基金项目： | 现代大豆产业技术体系资助项目，沈阳农业大学青年教师科研基金项目 |
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| 摘 要： | 研究了青霉素、甘氨酸、溶菌酶浓度、酶解温度、菌龄、再生培养基对生防芽孢杆菌Snb2原生质体的形成与再生的影响。培养液中青霉素和甘氨酸的终含量分别为0.3%和1.0%时,菌株Snb2的生长量最佳;菌株原生质体形成的最佳条件是,溶菌酶浓度为2.0mg·mL-1,酶解温度为35℃,生产原生质体的最佳菌龄为对数生长中期;在最佳再生培养基HCNB培养基上,原生质体再生率为66.0%。
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| 关 键 词： | 枯草芽孢杆菌 原生质体 制备 再生 影响因素 |
Production and Regeneration of Antagonistic Bacillus Snb2 Protoplast |
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| WANG Yuan-yuan,DUAN Yu-xi,CHEN Li-jie,ZHU Xiao-feng.Production and Regeneration of Antagonistic Bacillus Snb2 Protoplast[J].Journal of Shenyang Agricultural University,2011,42(1):110-113. |
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| Authors: | WANG Yuan-yuan DUAN Yu-xi CHEN Li-jie ZHU Xiao-feng |
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| Institution: | b (a.College of Biological Science and Technology,b.College of Plant Protection,Shenyang Agricultural University,Shenyang 110161,China) |
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| Abstract: | The effects of different factors,such as Pencillium,Glycin,Lysozyme dose,temperature,cell-age,regenerative medium on protoplast formation and regeneration of Bacillus subtilis Snb2 were studied.For strain Snb2 the growth medium for the final content of Pencillium and Glycin were 0.3%and 1.0% respectively for the best.The optimal condition was as followed: Lysozyme dose at 2.0mg/mL,temperature at 35℃,optimal cell-age was logarithmic growth mid-phase.On HCNB regenerative medium,the rate of protoplast regeneration was 66.0%. |
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| Keywords: | Bacillus subtilis protoplast formation regeneration affecting factors |
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