| 玉米顶腐镰孢菌rDNA-ITS和EF-1a基因序列及UP-PCR遗传多样性分析 |
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| 引用本文： | 陈璐,高增贵,庄敬华,张硕,高金欣.玉米顶腐镰孢菌rDNA-ITS和EF-1a基因序列及UP-PCR遗传多样性分析[J].沈阳农业大学学报,2011,42(1). |
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| 作者姓名： | 陈璐 高增贵 庄敬华 张硕 高金欣 |
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| 作者单位： | 沈阳农业大学,植物免疫研究所,沈阳,110161 |
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| 基金项目： | 国家科技支撑计划项目，国家粮丰工程项目，辽宁省教育厅重点实验室项目 |
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| 摘 要： | 对采自辽宁省部分地区的45份玉米顶腐病病株进行分离培养,共获得89株镰孢菌,采用传统形态学分类和现代分子生物学方法,共鉴定出4个种,分别为:亚粘团镰孢菌(Fusarium subglutinans)、轮枝镰孢菌(F.verticillioides)、层生镰孢菌(F.proliferatum)和尖孢镰孢菌(F.oxysporum).经EF-1a基因序列建立系统发育树,将顶腐镰孢菌分为4个组.用5条通用引物经UP-PCR扩增后,扩增出57条谱带,其中多态性条带53条,占总条带数的93.0%.遗传多样性分析表明,当相似系数0.626时,可将24个菌株划分为4个组,EF-1a基因序列与UP-PCR和ITS序列相比.更能体现镰孢菌种间和种内的亲缘关系及遗传差异性.
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| 关 键 词： | 玉米顶腐镰孢菌 EF-1α基因 通用引物PCR 遗传多样性 |
rDNA-ITS and EF-1a Gene Sequences of Fusarium species Causing Maize Top Rot and UP-PCR Analysis on Genetic Diversity |
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| CHEN Lu,GAO Zeng-gui,ZHUANG Jing-hua,ZHANG Shuo,GAO Jin-xin.rDNA-ITS and EF-1a Gene Sequences of Fusarium species Causing Maize Top Rot and UP-PCR Analysis on Genetic Diversity[J].Journal of Shenyang Agricultural University,2011,42(1). |
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| Authors: | CHEN Lu GAO Zeng-gui ZHUANG Jing-hua ZHANG Shuo GAO Jin-xin |
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| Institution: | CHEN Lu,GAO Zeng-gui*,ZHUANG Jing-hua,ZHANG Shuo,GAO Jin-xin (Institute of Plant Immunology,Shenyang Agricultural University,Shenyang 110161,China) |
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| Abstract: | A total of 89 Fusarium isolates were obtained from 45 diseased plants of maize top rot collected from some regions of Liaoning Province.Using traditional morphological classification and rDNA-ITS and EF-1a gene sequence analysis methods,4 Fusarium species,Fusarium.subglutinans,F.verticillioides,F.proliferatum and F.oxysporum,were identified.Twenty-three isolates were clustered into 4 groups based on EF-1a gene sequence on phylogenetic tree.Additionally,genetic diversity of Fusarium species was analyzed usin... |
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| Keywords: | ITS |
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