| 葡萄白藜芦醇合酶基因cDNA克隆及原核表达 |
| |
| 引用本文： | 付洪冰,崔莹,崔崇士.葡萄白藜芦醇合酶基因cDNA克隆及原核表达[J].沈阳农业大学学报,2011,42(4). |
| |
| 作者姓名： | 付洪冰 崔莹 崔崇士 |
| |
| 作者单位： | 1. 西安文理学院,西安,710065
2. 东北农业大学园艺系,哈尔滨,150030 |
| |
| 基金项目： | 哈尔滨市科技攻关计划项目(2003AA6CN088) |
| |
| 摘 要： | 从贝达葡萄中提取总RNA,采用RT-PCR方法,克隆到了白藜芦醇合酶(resveratrol synthase,RS)基因的全长cDNA。将其与圆叶葡萄(Vitis rotundifolia)、华东葡萄(Vitis pseudoreticulata)、河南毛葡萄(Vitis ficifolia)、河岸葡萄(Vitis vulpine)、酿酒葡萄(Vitis virufera)、羊蹄甲(Bauhinia)、花生(Arachis hvpogaea)、虎杖(Polygonum cuspiaatum)、大黄(Rheum officinale)进行氨基酸同源性比对,结果表明:分离的RS基因与其他葡萄的RS基因编码的氨基酸同源性达到97%以上,与其他属的RS基因编码的氨基酸同源性达到64%以上。生物信息学分析表明,该蛋白分子量为43kD,等电点pI=6.2,包含392个氨基酸残基。将RS的全长cDNA插入到表达载体pET28a中构建重组表达质粒pET28a-RS,转化大肠杆菌BL21,SDS-PAGE电泳检测结果表明,以30℃、0.5mmol.L-1IPTG诱导4h时该基因表达效果最好,诱导产物为1个约43kD的蛋白,为...
|
| 关 键 词： | 葡萄 白藜芦醇合酶 基因克隆 序列分析 原核表达 |
cDNA Sequence Analysis and Prokaryotic Expression of Resveratrol Synthase Gene in Vitis amurensis Rupr |
| |
| FU Hong-bing,CUI Ying,CUI Chong-shi.cDNA Sequence Analysis and Prokaryotic Expression of Resveratrol Synthase Gene in Vitis amurensis Rupr[J].Journal of Shenyang Agricultural University,2011,42(4). |
| |
| Authors: | FU Hong-bing CUI Ying CUI Chong-shi |
| |
| Institution: | FU Hong-bing1,CUI Ying1,CUI Chong-shi2 (1.Department of Life Sciences,Xi'an University of Arts and Science,Xi'an 710065,China,2.College of Horticulture,Northeast Agricultural University,Harbin 150030,China) |
| |
| Abstract: | The total RNA was extracted from leaves of Vitis Beida,and the full-length cDNA sequence of resveratrol synthase(RS) was cloned from the total RNA.Blast analysis of the amino acid sequence from the cDNA sequence of resveratrol synthase(RS) in NCBI database comparing to other varieties and other generas showed that the amino acid sequence was over 97% and 66% identity,respectively.In this paper,the protein product was analyzed using bioinformation and molecular biology software.The result indicated that the ... |
| |
| Keywords: | Vitis Beida resveratrol synthase gene cloning sequence analysis prokaryotic expression |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
