AtSUC3缺失对拟南芥愈伤组织分化及其蔗糖代谢的影响

以拟南芥愈伤组织为材料,研究了拟南芥蔗糖转运蛋白基因AtSUC3经T-DNA插入突变对拟南芥愈伤组织的分化及其蔗糖代谢的影响。试验结果表明:愈伤组织出现的时间早于atsuc3,且生长略快于atsuc3,二者质地均疏密适中;在蔗糖浸泡液中的果糖和葡萄糖不是由愈伤组织外渗的,而是转化酶作用于培养基中蔗糖的结果;野生型愈伤组织的蔗糖转化酶活性略高于atsuc3愈伤组织,野生型愈伤组织浸泡液中蔗糖的浓度始终低于atsuc3,而浸泡液中的葡萄糖浓度高于atsuc3。因此,AtSUC3缺失使拟南芥愈伤组织的分化和生长受阻,但影响不大;atsuc3愈伤组织对蔗糖的吸收能力弱于野生型;AtSUC3T-DNA插入突变体由于对蔗糖的吸收能力降低,而增加对葡萄糖的吸收,以作为对碳源的补偿。

Influence of the Identification of AtSUC3 on the Differentiation of Callus and Sucrose Metabolization of Arabidopsis thaliana

Taking the callus of Arabidopsis thaliana as material,the paper explored the influence of AtSUC3 after inserted by T-DNA on the callus differentiation and sucrose metallization.Wild-type callus appeared before atsuc3,and grow faster than the latter;their densities were moderate.Fructose and glucose in sucrose solution were not filtered by callus,but caused by the invertase.The invertase activity of the wild-type was higher than that of atsuc3.Sucrose content of wild-type callus was lower than that of atsuc3 and glucose content of wild-type callus was higher than that of atsuc3.The results showed that the absence of AtSUC3 delayed the differentiation and growth of callus.The absorption of sucrose of atsuc3 callus was weaker than that of the wild-type.Because of the reduction of ability to absorb sucrose,atsuc3 had to increase the absorption of glucose to compensate the loss of carbon source.