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| 高粱丝黑穗病抗病基因SSR分析 | |
| 引用本文： | 李玥莹,王天舒,邹剑秋,李南珠. 高粱丝黑穗病抗病基因SSR分析[J]. 沈阳农业大学学报, 2008, 39(6) |
| 作者姓名： | 李玥莹 王天舒 邹剑秋 李南珠 |
| 作者单位： | 1. 沈阳师范大学化学与生命科学学院,沈阳,110034 2. 中国农业大学生物学院,北京,100083 3. 辽宁省农业科学院,沈阳,110161 4. 沈阳市中医药学校,沈阳,110300 |
| 基金项目： | 辽宁省教育厅资助项目 ， 公益性行业(农业)科研专项经费资助项目 |
| 摘 要： | 为了筛选高粱丝黑穗病抗病基冈的SSR标记,以高粱抗病亲本(7050B、2381R)和感病亲本(Tx622B、矮四),及其杂交组合后代为材料,用CTAB法从叶片中提取高粱基因组DNA,通过SSR技术对高粱丝黑穗病3号生理小种抗性基因进行了初步筛选.结果表明:供试109对SSR引物中筛选出扩增条带清晰且稳定的引物94对.其中1个SSR位点Xtxp80与高梁丝黑穗病3号生理小种抗病基因相关.采用Mapmarker软件,对2381Rx矮四组合的98个F2,单株的SSR扩增结果进行了分析,初步确定高粱丝黑穗病3号生理小种抗性基因与SSR位点Xtxp80连锁,距离为8.3cM. |
| 关 键 词： | 高粱 丝黑穗病 抗病基因 |
SSR Analysis of Sorghum Resistance to Head Smut |
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| LI Yue-ying,WANG Tian-shu,ZOU Jian-qiu,LI Nan-zhu. SSR Analysis of Sorghum Resistance to Head Smut[J]. Journal of Shenyang Aricultural University, 2008, 39(6) | |
| Authors: | LI Yue-ying WANG Tian-shu ZOU Jian-qiu LI Nan-zhu |
| Affiliation: | LI Yue-ying1,WANG Tian-shu2,ZOU Jian-qiu3,LI Nan-zhu4(1.College of Chemistry , Life Science,Shenyang Normal University,Shenyang 110034,China,2.College of Biological Science,China Agricultural University,Beijing 100083,3.Liaoning Academy of Agricultural Sciences,Shenyang 110161,4.Shenyang Traditional Chinese Medicine School,Shenyang 110300,China) |
| Abstract: | The objectives of the research were to screen molecular markers of sorghum resistant gene to the physiological strain No.3 of head smut with SSR.Bulked Segregation Analysis(BSA) was applied in the study.Two segregation populations,restorer line population(R population) 2381R/Aisi and maintain line population(B population) Tx622B/7050B,were used in the research.The amplification bands of 94 pairs primers from 109 ones were clear and stable.The amplification bands showed that only one SSR locus Xtxp80 related... |
| Keywords: | SSR |
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