| 两种聚丙烯酰胺凝胶银染方法的比较 |
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| 引用本文： | 关海涛,徐世昌,郭玉华.两种聚丙烯酰胺凝胶银染方法的比较[J].沈阳农业大学学报,2006,37(1):86-87. |
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| 作者姓名： | 关海涛 徐世昌 郭玉华 |
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| 作者单位： | 1. 沈阳农业大学,农学院,沈阳,110161
2. 中国农业科学院,北京,100094 |
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| 基金项目： | 国家“973”计划项目(G2000016200)，国家“863”计划项目(2002AA245041)，国家自然科学基金项目(30270867及30471131) |
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| 摘 要： | 比较了两种聚丙烯酰胺凝胶的DNA染色方法。对小麦SSR扩增产物的染色结果显示,改进的Bassam银染法颜色背景浅,灵敏度较高,能够看到副带染色。改进的Sanguinetti方法对条带的鉴别力稍差,但因省时省力,成本较低可用于大规模引物筛选时的染色。
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| 关 键 词： | 银染法 聚丙烯酰胺电泳 SSR 扩增产物 小麦 |
| 文章编号： | 1000-1700(2006)01-0086-02 |
| 收稿时间： | 2004-09-16 |
| 修稿时间： | 2004年9月16日 |
Comparison of Two Ways of Silver Staining for DNA in PAGE |
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| GUAN Hai-tao,XU Shi-chang,GUO Yu-hua.Comparison of Two Ways of Silver Staining for DNA in PAGE[J].Journal of Shenyang Agricultural University,2006,37(1):86-87. |
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| Authors: | GUAN Hai-tao XU Shi-chang GUO Yu-hua |
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| Institution: | 1.CoUege of Agronomy, Shenyang Agricultural University, Shenyang 110161,China; 2. Chinese Academy of Agricultural Sciences, Beijing 100094,China |
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| Abstract: | Two ways of DNA silver staining for PAGE were compared.The results showed that the background color was shallower and the SSR band of wheat was clearer when the improved method of Bassam was used compared with the improved method of Sanguinetti.The latter can be used when the large-scale primers are screened for saving time and work,though it is not sensitive. |
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| Keywords: | silver staining PAGE SSR amplified products wheat |
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