|
|||||
|
|
| 检测肥育猪CAST蛋白的免疫印迹方法研究 | |
| 引用本文： | 张勇,傅刚,翟峰,朱宇旌,孙景霞,邵彩梅.检测肥育猪CAST蛋白的免疫印迹方法研究[J].沈阳农业大学学报,2011,42(3):321-324. |
| 作者姓名： | 张勇 傅刚 翟峰 朱宇旌 孙景霞 邵彩梅 |
| 作者单位： | 1. 沈阳农业大学畜牧兽医学院,沈阳,110161 2. 沈阳大学,沈阳,110044 3. 大连老虎滩海洋公园鸟语林,辽宁大连,116013 4. 辽宁禾丰牧业股份有限公司,沈阳,110164 |
| 基金项目： | 国家自然科学基金项目(30500363) |
| 摘 要： | 为了研究3个不同蛋白质水平(10%、18%、26%)饲粮对肥育猪的钙蛋白抑制蛋白酶(CAST)的影响,对CAST蛋白提取、电泳、转膜等条件进行优化。试验表明:CAST被激活的条件是将蛋白提取液于上样缓冲液95℃煮15min后,立即在冰块上冷却,防止蛋白降解;采用分离胶浓度为10%进行电泳,可以明显检测到68kD、107 kD的CAST;采用预染Marker和DAB染色液可针对性地检测到目的蛋白的位置;利用上述程序检测到了猪CAST两种类型的表达,取得了较满意的结果。 |
| 关 键 词： | CAST 免疫印迹 肥育猪 |
Study on Western Blot Method to Detect CAST in Growing-Finishing Pigs |
|
| ZHANG Yong,FU Gang,ZHAI Feng,ZHU Yu-jing,SUN Jing-xia,SHAO Cai-mei.Study on Western Blot Method to Detect CAST in Growing-Finishing Pigs[J].Journal of Shenyang Agricultural University,2011,42(3):321-324. | |
| Authors: | ZHANG Yong FU Gang ZHAI Feng ZHU Yu-jing SUN Jing-xia SHAO Cai-mei |
| Institution: | ZHANG Yong1,FU Gang2,ZHAI Feng1,ZHU Yu-jing1,SUN Jing-xia3,SHAO Cai-mei4 (1.College of animal science and veterinary medicine,Shenyang Agricultural University,Shenyang 110161,China,2.Shenyang University,Shenyang 110044,3.Bird-singing Woods,Dalian Laohutan Ocean Park,Dalian,Liaoning 116013,4.Liaoning Wellhope Agri-Tach Co.Ltd,Shenyang 110164,China) |
| Abstract: | Western blotting method to investigate effects of different dietary levels protein(10%,18% and 26%) on the expression of CAST of growing-finishing pigs was studied.Its procedures including the extraction of protein,the condition of electrophoresis and immuno-blotting were optimized.The results showed that CAST was activated after the extract was heated at 95℃ for 15min and then cooled on the ice immediately in order to prevent being degraded.CAST of 68 kD and 107 kD was detected by using a separation gel of 10% for SDS-PAGE preparation,the target protein position was detected directly by pre-stained Marker and DAB staining solution.Two types of CAST were detected successfully by the above procedures. |
| Keywords: | CAST immunoblotting finishing pigs |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |