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牡丹组织培养中褐化的发生原因与防止方法的研究
引用本文:张俊琦,罗晓芳.牡丹组织培养中褐化的发生原因与防止方法的研究[J].沈阳农业大学学报,2006,37(5):720-724.
作者姓名:张俊琦  罗晓芳
作者单位:北京林业大学,生物科学与技术学院,北京,100083
基金项目:北京林业大学人才振兴计划资助项目(200203084)
摘    要:在牡丹组织培养过程中,褐化现象普遍发生,并影响着牡丹培养物的正常生长与增殖。因此通过改变培养条件、培养方式以及在培养基中添加抑褐剂的方法,对品种为青龙卧墨池的牡丹组培物褐化的防止进行了研究,结果表明:添加生长素会加剧启动培养中外植体的褐化程度,暗培养对愈伤组织抑制褐化很有效,悬浮培养未能减缓褐化却能使愈伤快速增殖。吸附剂聚乙烯吡咯烷酮(PVP)和结冷胶(Phytagel)能够有效地缓解褐化。抑褐剂硫代硫酸钠(Na2S2O3)、抗坏血酸(VC)和铜试剂(DDTC)在牡丹的组培中加剧了组织培养物的褐化程度。

关 键 词:牡丹  组织培养  褐化现象  抑褐剂  外植体
文章编号:1000-1700(2006)05-0720-05
收稿时间:2005-10-20
修稿时间:2005年10月20

Occurrence and Prevention Method of Brown Turning in Tissue Culture of Peonia suffruticosa Andr
ZHANG Jun-qi,LUO Xiao-fang.Occurrence and Prevention Method of Brown Turning in Tissue Culture of Peonia suffruticosa Andr[J].Journal of Shenyang Agricultural University,2006,37(5):720-724.
Authors:ZHANG Jun-qi  LUO Xiao-fang
Institution:College of Biological Sciences and Biotechnology, Beifing Forestry University, Beijing 100083, China
Abstract:Browning takes place generally in tissue culture of peony and becomes serious during the whole processes,so the growth of cultures was affected.By means of changing culture environment and method,appending anti-browning into the culture medium,to prevent the browning of cultures of P.suffruticosa cv.Qinglongwomochi was carriedon.The results showed that adding auxin into the culture medium accelerated the cultures browning in the initial stage of the aseptic culture.Dark culture was most effective to prevent callus browning,the liquid suspending culture proliferated callus quickly though not prevent the browning effectively.The adsorbent PVP and Phytagel performed an effective action in alleviating browning.However,the anti-oxidant,such as Na_2S_2O_3,VC,and DDTC were ineffective to diminish the browning,even turned worse the degree of browning.
Keywords:Peonia suffruticosa Andr  tissue culture  browning  anti-browning chemicals  explant
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