激光切割对马铃薯组培苗移栽后生长指标的影响

以机械剪切方式作为对照,使用以CO_2激光器在垂直照射、离焦量12 mm、光输出功率10 W的条件下的激光,对马铃薯组培苗进行无接触式切割,并对激光切割和机械剪切后马铃薯组培苗的生长情况进行对比试验。试验结果显示:使用激光进行马铃薯组培苗切割不会影响其生物活性;在培养21 d后,无论是单切口茎段还是双切口茎段,激光切割马铃薯组培苗的存活率、株高、节间数、节间长度、茎粗、根长、去根鲜质量等生长指标均优于机械剪切方式。试验结果表明,以激光切割方式代替传统机械剪切方式进行马铃薯组培苗的无接触式切割,极大简化了组培苗移栽时的动作,能有效避免组培苗交叉感染,促进移栽的组培苗茎段成活。

Effect of laser cutting on growth indexes of potato tissue cultured seedlings after transplanting

At present,the method of cutting potato tissue culture seedling is still using scissors to cut. This method has low efficiency of production and is easy to cause virus infection,which makes it difficult to control the quality of transplanting seedling. To solve the problems mentioned above,using laser to cut potato tissue culture seedlings without contact was proposed. The growth of potato tissue culture seedlings after laser cutting and mechanical shearing was compared. The experimental group used CO2laser to cut potato tissue culture seedlings under the conditions of vertical irradiation,defocus of 12 mm and light output power of 10 W,while the control group used mechanical shearing. Results showed that potato tissue culture seedlings cut with laser did not affect its biological activity. After 21 days of culture,the survival rate,plant height,number of internodes,internode length,stem diameter,root length and fresh weight of root removal of laser cutting were better than those of mechanical shearing for both single-incision and double-incision stems. It is indicated that the non-contact cutting of potato tissue culture seedlings by laser cutting instead of traditional mechanical shearing greatly simplifies the transplantation of tissue culture seedlings,effectively avoids cross-infection of the tissue culture seedlings,and promotes the stem survival of transplanted tissue culture seedlings.