地方鸡禽白血病病毒感染调查及主要流行亚群分析

为了解湖北省地方鸡禽白血病病毒的感染状态及主要流行亚群,从麻城绿壳蛋鸡、江汉鸡、景阳鸡3个地方品种核心群采集了7 477份样品,进行蛋清p27抗原检测、公鸡病毒分离鉴定及分离株gp85基因序列分析。结果显示:3个地方鸡种蛋清p27抗原阳性率分别为1.32%、6.54%和2.82%,公鸡病毒分离率为6.11%、30.14%和8.64%,表明湖北省地方鸡禽白血病病毒感染比较严重,不同品种的感染率不同,公鸡的排毒率高于母鸡。利用PCR方法将分离的15株禽白血病病毒分为两大亚群,分离株gp85基因遗传进化分析结果显示,3个地方鸡品种主要流行J、K亚群禽白血病病毒,均存在J、K亚群禽白血病病毒的共感染,表明湖北省地方鸡禽白血病病毒感染情况较为复杂。

Investigation of avian leukosis virus infection and analysis of main epidemic subgroups in indigenous chicken

In order to understand the infection status and main epidemic subgroups of avian leukosis virus (ALV) in indigenous chicken of Hubei province,7 477 samples were collected from three local core breeds groups:the Macheng green-shell layer chicken,the Jianghan chicken and the Jingyang chicken groups,followed by the detection of the egg white p27 antigen,isolation and identification of ALV from roosters,and sequence analysis of the gp85 gene. The results showed that the positive rate of p27 antigen in the three breed groups were 1.32%,6.54% and 2.82%,respectively,and the isolation rates of ALV from roosters were 6.11%,30.14% and 8.64%,respectively,indicating that the ALV infection rate in chickens in Hubei province was relatively serious. The 15 ALV isolates can be divided into two subsets using PCR. Genetic evolution analysis of the gp85 gene showed that the J subgroup (ALV-J) and K subgroup (ALV-K) were prevalent in the three indigenous chicken breed groups,and co-infection of ALV-J and ALV-K was also found in all the three groups. The results indicated that the ALV infection in indigenous chickens in Hubei province was complicated.