海带岩藻聚糖硫酸酯对四氯化碳致肝损伤小鼠的保护作用

对小鼠分别灌胃200、400、800 mg/(kg·d)的海带岩藻聚糖硫酸酯(简称FLJ,利用酶解法制备),于第7天腹腔注射四氯化碳制备小鼠急性肝损伤模型,以200 mg/(kg·d)的联苯双酯做阳性对照.测定血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)活性及肝组织中丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性,并观察肝组织形态学变化,研究FLJ对CCl_4诱导急性肝损伤小鼠的保护作用.结果表明:FLJ各剂量均能抑制肝损伤小鼠血清ALT、AST活性的升高,且中、高剂量组达到显著水平,并能显著提高肝组织中SOD活力、降低MDA含量,减轻由对肝细胞的病理损伤.FLJ对CCl_4造成的小鼠急性肝损伤具有一定的保护作用.

Hepatoprotective Activity of Fucoidan from Laminaria japonica against CCl4 Induced Hepatic Injury in Mice

Fucoidan was prepared from Laminaria japonica by enzymatic hydrolysis.Mice were pretreated with fucoidan from Laminaria japonica(200,400,800 mg/kg respectively for 7 days).Then liver injury model was established by administration of CCl_4 in mice on the 7th day.The positive control was administrated with 200 mg/kg dimethyl diphenyl bicarboxylate.The activities of serum ALT and AST,the content of MDA and the activity of SOD in hepatic tissue were measured.The hepatic histological changes were observed by optical microscope.The results showed that fucoidan from Laminaria japonica had significantly hepatoprotective activity on CCl_4 induced liver damage in mice.Mice treated with fucoidan remarkably prevented the elevation of serum AST,ALT and liver lipid peroxides in CCl_4 treated mice(P<0.01).Histopathological changes induced by CCl_4 were also significantly reduced by fucoidan treatment.