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盆栽安祖花叶片愈伤组织诱导及植株再生的影响因素
引用本文:刘国锋,赵庆庆,包满珠.盆栽安祖花叶片愈伤组织诱导及植株再生的影响因素[J].华中农业大学学报,2009,28(3).
作者姓名:刘国锋  赵庆庆  包满珠
作者单位:华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室,武汉,430070
基金项目:国家高技术研究发展计划(863计划),华中农业大学引进人才科研启动基金 
摘    要:以近几年新引进的6个安祖花盆栽品种为试材.以刚展开的幼嫩叶片为外植体,研究了基本培养基、植物生长调节剂配比、培养条件等因素对其愈伤组织诱导及不定芽分化、增殖的影响.结果表明:MP和1/2MS培养基比MS、B5和N6培养基更适于愈伤组织的诱导,1.0~2.0 mg/L BA+0.1~0.2 mg/L 2,4-D对多数品种的愈伤诱导较为合适,MS+O.5 mg/L BA+0.5 rng/L NAA和MP+1.0 mg/L BA适于参试品种的不定芽分化,N6培养基不利于芽的分化;不定芽的增殖以MS+1.0 mg/L BA最为合适.不同品种的愈伤组织诱导能力、不定芽分化能力及其增殖效率差异明显.黑暗培养比光照培养更有利于愈伤组织的诱导.理想的生根培养基和移栽基质分别为1/2 MS+0.5 mg/L IAA和泥炭土与珍珠岩的混合物,生根率与移栽成活率均可达100%.

关 键 词:安祖花  盆栽品种  基因型  植株再生  组织培养

Factors Affecting Callus Induction and Plant Regeneration from Leaf Explants of Pot Anthurium(Anthurium andraeanum Lind.)
LIU Guo-feng,ZHAO Qing-qing,BAO Man-zhu.Factors Affecting Callus Induction and Plant Regeneration from Leaf Explants of Pot Anthurium(Anthurium andraeanum Lind.)[J].Journal of Huazhong Agricultural University,2009,28(3).
Authors:LIU Guo-feng  ZHAO Qing-qing  BAO Man-zhu
Institution:Key Laboratory of Horticultural Plant Biology;Ministry of Education/College of Horticultureand Forestry Sciences;Huazhong Agricultural University;Wuhan 430070;China
Abstract:Using immediately expanded blades as explants,the effects of different basic media,PGR(plant growth regulator) combinations and illumination conditions on the callus induction and adventitious shoot differentiation and proliferation were investigated in six pot cultivars of Anthurium andraeanum.The results showed that MP and 1/2 MS media were better than the MS,B5 or N6 medium for callus induction.1.0~2.0 mg/L BA plus 0.1~0.2 mg/L 2,4-D were suitable for callus induction of most cultivars investigated.MS+0....
Keywords:Anthurium andraeanum  pot cultivars  genotype  plant regeneration  tissue culture  
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