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| 不同靶点shRNA干扰对虾白斑综合征病毒增殖效果分析 | |
| 引用本文： | 周俊芳,杨先乐,万夕和,沈 辉,房文红.不同靶点shRNA干扰对虾白斑综合征病毒增殖效果分析[J].华中农业大学学报,2011,30(1):105-108. |
| 作者姓名： | 周俊芳 杨先乐 万夕和 沈 辉 房文红 |
| 作者单位： | 1. 中国水产科学研究院东海水产研究所/农业部海洋与河口渔业资源及生态重点开放实验室,上海,200090 2. 上海海洋大学农业部渔业动植物病原库,上海,201306 3. 江苏省海洋水产研究所,南通,226007 |
| 基金项目： | 中国博十后科学基金项目，中央级公益性科研院所基本科研业务费专项 |
| 摘 要： | 设计合成了靶向对虾白斑综合征病毒(WSSV)基因组不同佗点的5个shRNA:RR9、RR1、DP1、DP2和PK1,并在凡纳滨对虾体内实施了RNA干扰(RNAi)抗WSSV增殖的试验.结果显示:5个shRNA不同程度地抑制了WSSV的复制,降低了对虾的死亡率,其中,靶向病毒核糖核酸还原酶的shRNA RR9的RNA干扰效果最佳. |
| 关 键 词： | 凡纳滨对虾 对虾白斑综合征病毒 RNA干扰 |
| 收稿时间： | 6/8/2010 12:00:00 AM |
Effect of the viral interference effects of shRNAs targeting different sites in white spot syndrome virus genome |
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| ZHOU Jun-fang,YANG Xian-le,WAN Xi-he,SHEN Hui,FANG Wen-hong.Effect of the viral interference effects of shRNAs targeting different sites in white spot syndrome virus genome[J].Journal of Huazhong Agricultural University,2011,30(1):105-108. | |
| Authors: | ZHOU Jun-fang YANG Xian-le WAN Xi-he SHEN Hui FANG Wen-hong |
| Institution: | ZHOU Jun-fang1 YANG Xian-le2 WAN Xi-he3 SHEN Hui3 FANG Wen-hong11.East China Sea Fisheries Research Institute Chinese Academy of Fishery Sciences/Key Laboratory of Marine and Estuarine Fisheries Resources and Ecology,Ministry of Agriculture,Shanghai 200090,China,2.Aquatic Pathogen Collection Center of Ministry of Agriculture,Shanghai Ocean University,Shanghai 201306,3.Jiangsu Institute of Marine Fisheries,Nantong 226007 |
| Abstract: | Five shRNAs targeting different sites in white spot syndrome virus(WSSV) genome,respectively designated as RR9,RR1,DP1,DP2 and PK1,were designed and constructed.Followed by WSSV infection,each of which was introduced into Litopenaeus vannamei shrimp by injection.The result demonstrated that both the proliferation of WSSV in vivo and the shrimp mortality were decreased in different degrees in all of the shRNAs-treated groups.Based on the analysis,the RR9 targeting WSSV ribonucleotide reductase gene had the b... |
| Keywords: | Litopenaeus vannamei white spot syndrome virus(WSSV) RNA interference(RNAi) |
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