| 猪环状病毒2型的PCR检测方法的建立及应用 |
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| 引用本文： | 曹胜波,陈焕春,等.猪环状病毒2型的PCR检测方法的建立及应用[J].华中农业大学学报,2001,20(1):53-56. |
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| 作者姓名： | 曹胜波 陈焕春 |
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| 作者单位： | 华中农业大学畜牧兽医学院, |
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| 摘 要： | 根据国外已发表的猪环状病毒2型（PCV－2）的全基因组序列，设计一对2型特异性引物，对可疑病料进行PCR扩增。将扩增产物连接到pMD18－T载体上并克隆到大肠杆菌DH5α中，经提取质粒进行PCR、酶切、测序鉴定，证明扩增出了PCV－2的目的片段。将测序结果与GenBank收录的PCV－2序列进行比较，发现同源性均在90％以上。用该PCR方法在43份可疑病料中检测到了12分PCV－2阳性病料，表明该病在我国已有流行。
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| 关 键 词： | 猪环状病毒2型 检测方法 聚合酶链反应 |
| 修稿时间： | 2000年11月14 |
The Establishment And Application of PCR to Diagnosis Porcine Circovirus Type 2 |
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| Cao Shengbo,Chen Huanchun,Xiao Shaobo,He Qigai,Xu Yindi,Liu Junfa.The Establishment And Application of PCR to Diagnosis Porcine Circovirus Type 2[J].Journal of Huazhong Agricultural University,2001,20(1):53-56. |
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| Authors: | Cao Shengbo Chen Huanchun Xiao Shaobo He Qigai Xu Yindi Liu Junfa |
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| Abstract: | According to the published genome sequences of porcine circovirus type 2 (PCV-2) a pair of specialprimers was designed to detect PCV-2 in samples from some farms by PCR. The PCR product was extracted and linked to pMD 18-T vector and then was colned into E.coli.DH5α.The recombination plasmid was identified by digestion with different enzyme restrictions, PCR and sequencing analysis. The homology of nucleotide sequence between the PCR product and other PCV-2 strains was more than 90%. Within forty three samples, twelve positive samples were found by PCR. It showed that PCV-2 infection is popular in our country. |
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| Keywords: | porcine circovirus type 2 diagnosis polymerase chain reaction(PCR) |
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