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油菜内生真菌的分离鉴定及抑茵作用
引用本文:兰 楠,祁高富,喻子牛,赵秀云.油菜内生真菌的分离鉴定及抑茵作用[J].华中农业大学学报,2011,30(3):270-275.
作者姓名:兰 楠  祁高富  喻子牛  赵秀云
作者单位:华中农业大学生命科学技术学院/农业微生物学国家重点实验室,武汉,430070
基金项目:国家"863"计划项目
摘    要:从油菜幼苗中通过PDA培养基分离出9株内生真菌,通过形态观察和18S rRNA基因序列分析,其中6株鉴定为链格孢菌(Ahernaria alternata),1株鉴定为球毛壳菌(Chaetomium globosum),1株属于镰刀菌属(Fusarium),1株属于炭角菌目(Xylariales).从鉴定出的油菜内生真...

关 键 词:内生真菌  18S  rRNA  球毛壳菌  抑菌作用
收稿时间:4/7/2010 12:00:00 AM

Isolation,identification and anti-fungal action of endophytic fungi of rapeseed
LAN Nan,QI Gao-fu,YU Zi-niu,ZHAO Xiu-yun.Isolation,identification and anti-fungal action of endophytic fungi of rapeseed[J].Journal of Huazhong Agricultural University,2011,30(3):270-275.
Authors:LAN Nan  QI Gao-fu  YU Zi-niu  ZHAO Xiu-yun
Abstract:Nine strains of endophytic fungi were isolated from rapeseed and classified into 4 genera through morphological and molecular biological identification.Plate confronting test showed that the endophytic Chaetomium globosum had different inhibition on Sclerotinia sclerotiorum,Rhizoctonia solani,Botrytis cinerea and Fusarium graminearum,etc.The mechanism of antagonism C.globosum and R.solani with confrontation on slide found that C.globosum had apparent process of mycoparasitism during the confrontation with R.solani.
Keywords:18S rRNA
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