甘蓝型油菜与甘蓝种间杂种的鉴定及特性研究

采用新型甘蓝型油菜细胞质雄性不育源(Eru CMS)为材料,通过杂交与胚抢救技术,获得甘蓝型油菜与甘蓝的种问杂种,对杂种进行鉴定并对杂种性状进行研究.用流式细胞仪和染色体计数鉴定后发现,真杂种的DNA含量为两亲本的中间值,并且染色体数目均为28条.通过对真杂种特性进行研究,结果表明:杂种植株幼苗形态都介于亲本之间,生长...

Identification and characterization of interspecific hybrids from Brassica napus(Eru CMS)×Brassica oleracea

Hybrids from Brassica napus×Brassica oleracea were obtained by embryo rescue techniques,which is an effective approach for increasing the resources of male sterility of Brassica oleracea.Both the DNA content and chromosome number of hybrids were the mid-parent values.Characteristics of the hybrid were investigated.The results showed that hybrids were morphologically similar to parents during the growing periods,while inclined to the female parent during flowering.But hybrids had strong heterosis of growth.The leaf structure,chloroplast number in stomata guard cell,the floral organ,and the early flowering periods of hybrids were mid-parent,except the mutated leaf structure of mosaic hybrid.Hybrids had their own unique peroxidase isozymes bands.Test of the pollen viability showed that hybrids bore only non-viability pollens with 100% sterility.Backcross generation could be obtained only through embryo rescue techniques to overcome barriers of hybridization.