| 黑曲霉木聚糖酶基因（xynA）在大肠杆菌中的表达及酶学分析 |
| |
| 引用本文： | 崔罗生,祝茂生,徐顺清,朱辉,梁运祥,张忠明.黑曲霉木聚糖酶基因（xynA）在大肠杆菌中的表达及酶学分析[J].华中农业大学学报,2009,28(1). |
| |
| 作者姓名： | 崔罗生 祝茂生 徐顺清 朱辉 梁运祥 张忠明 |
| |
| 作者单位： | 华中农业大学生命科学技术学院,武汉,430070 |
| |
| 基金项目： | 浙江省重大科技攻关项目 |
| |
| 摘 要： | 从黑曲霉(Aspergillus niger)F19中克隆得到木聚糖酶基因xynA.将不带原基因信号肽编码序列的xynA基因段以正确的阅读框架克隆到大肠杆菌(Escherichia coli)表达载体pET-28a(+)上,并转化E.co-li BL21,获得重组工程菌BLX1.经过IPTG诱导,xynA获得特异性表达,其表达产物以包涵体和胞内可溶性蛋白2种形式存在.经过SDS-PAGE分析,重组蛋白分子质量约为24 ku.酶学分析表明,最适反应温度为50℃,最适反应pH为5.0,在酸性条件下具有较好的稳定性.
|
| 关 键 词： | 黑曲霉 木聚糖酶 xynA基因 酶学性质 |
Expression of Xylanase Gene xynA from Aspergillus niger F19 in the Escherichia coli and Its Enzymatic Characterization |
| |
| CUI Luo-sheng,ZHU Mao-sheng,XU Shun-qing,ZHU Hui,LIANG Yun-xiang,ZHANG Zhong-ming.Expression of Xylanase Gene xynA from Aspergillus niger F19 in the Escherichia coli and Its Enzymatic Characterization[J].Journal of Huazhong Agricultural University,2009,28(1). |
| |
| Authors: | CUI Luo-sheng ZHU Mao-sheng XU Shun-qing ZHU Hui LIANG Yun-xiang ZHANG Zhong-ming |
| |
| Institution: | College of Life Science and Technology;Huazhong Agricultural University;Wuhan 430070;China |
| |
| Abstract: | The gene xynA encoding xylanase was cloned from the Aspergillus niger F19.The xynA without its origin signal peptide sequence was fused into the Escherichia coli expression vector pET-28a(+) with correct open reading frame,then transfered into the E.coli BL21.The recombinant strain BLX1 was finally obtained.Induced by IPTG,the xynA gene was specifically expressed in the recombinant strain BLX1.The expression product existed as both inclusion bodies and intracellular soluble forms.The molecular weight of the... |
| |
| Keywords: | Aspergillus niger xylanase xynA gene enzymatic characterization |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《华中农业大学学报》浏览原始摘要信息 |
| 点击此处可从《华中农业大学学报》下载免费的PDF全文 |
|
