春夏季节东湖不同富营养化区域水体细菌群落的动态变化

采用高通量测序技术,对东湖2个不同富营养化区域(官桥湖区和海洋世界区,简称H区和L区)在春夏季节水体细菌群落的动态变化进行了研究。结果表明:H区和L区细菌的群落结构相似,放线菌门(Actinobacteria)占优势,其次为蓝藻门(Cyanobacteria)和变形菌门(Proteobacteria)。2个区域细菌的相对丰度随时间的变化出现差异,在门水平上,H区的蓝藻门(10.2%~33.7%)与放线菌门(15.4%~53.1%)波动较大,且两者呈显著负相关,L区则相对稳定;在纲水平上,H区波动明显的是放线菌纲(Actinobacteria)、集球藻亚纲(Synechococcophycideae)、颤藻亚纲(Oscillatoriophycideae)和Chloroplast,L区出现波动的是颤藻亚纲和Chloroplast。L区的多样性指数高于H区。通过RDA分析发现,pH值、水温、溶解氧与2个区域的细菌群落有较强的负相关关系,氮磷比和总磷分别与H区和L区的细菌群落有较强的正相关关系。

Dynamic changes of bacterial community in two eutrophic areas of Donghu Lake in spring and summer

The dynamic changes of bacterial community in two eutrophic areas including Guanqiao Lake and Sea World,referred to as H and L,of Donghu Lake in spring and summer were studied with high throughput sequencing. The results showed that the bacterial community structures in H and L areas were similar.Actinobacteria were dominated in these area,following by Cyanobacteria and Proteobacteria. The relative bacterial abundance varied with time in both areas tested. A large fluctuation was observed in H area that Cyanobacteria (10.2%-33.7%) and Actinobacteria (15.4%-53.1%) abundance varied with significantly negative correlation,while bacteria were relatively stable in L area at the phylum level. The abundance of several classes including Actinobacteria,Synechococcophycideae,Oscillatoriophycideae and Chloroplast in H area,and the Oscillatoriophycideae and Chloroplast in L area fluctuated greatly. The diversity index of L area was higher than that of H area. Results of RDA analyses showed that pH,water temperature and dissolved oxygen were significantly negatively correlated with bacterial community in both areas,while N/P and TP were strongly positively correlated with bacterial community of H and L areas.