饲粮水平对猪IMF含量、脂肪酸合成酶mRNA表达量及血液激素含量的影响

试验选用84头10 kg左右杜洛克×长白×大约克(DLY)三元杂交阉公猪,随机分成2组,组内设6个重复,每个重复7头猪,研究了在NRC(1998)标准(对照组)、荣昌猪(GB 7223-1987)饲养标准(试验组)2种饲粮条件下营养水平对DLY猪生长肥育期背最长肌I MF含量、HSL及FAS mRNA表达量和血液激素含量的影响。结果表明:降低饲粮营养水平可降低35、80及110 kg DLY猪血清胰岛素含量和35及80 kg猪生长激素含量;同时可升高20、50及110 kg DLY猪血清肾上腺素含量和35、50及110 kg DLY猪胰高血糖素含量。各阶段试验组背最长肌肌内脂肪含量均高于对照组,DLY猪50 kg时达到了显著水平;除50 kg组外,其余各阶段试验组背最长肌HSL mRNA及FAS mRNA表达量均高于对照组;降低饲粮营养水平有升高20、35及80 kg DLY猪FAS mRNA/HSL mRNA的趋势。

Effect of Nutrition Levels of Diet on IMF Concentration, Fatty Acid Synthase mRNA in longissimus dorsi and Serum Hormones of Pigs during Growing-finishing Period

Eighty-four healthy Duroc Landrace Yorkshire barrows(10 kg body weight) were allocated to control(NRC.1998,nutrient requirements of swine) or treatment group(GB 7223-1987,nutrient requirements of Rongchang swine),each group having 6 replicates comprising 7 pigs each,until 110 kg body weight to determine IMF concentration,HSL,FAS mRNA in longissimus dorsi and hormones in serum of DLY during growing-finishing period.The results showed that:As dietary nutritional level declined,the concentration of insulin in serum was decreased when body weight was 35,80 and 110 kg(P>0.05),and that of somatotropin in serum was also reduced when body weight was 35 and 80 kg(80 kg,P<0.01).At the same time,the concentration of epinephrine is serum when body weight was 20,50 and 110 kg(110 kg,P<0.05),and glucagon were increased when body weight was 35,50 and 110 kg (50 and 110 kg,P<0.05).Intramuscular fat in longissimus dorsi of treatment was higher than that of control during growing-finishing period,and it was significant when body weight was 50 kg(P<0.05).HSL mRNA and FAS mRNA in longissimus dorsi of treatment was higher than that of control except that body weight was 50 kg(20 kg,P<0.01;110 kg,P<0.05).FAS mRNA/HSL mRNA had a tendency of increase as dietary nutritional level declined when body weight was 20,35 and 80 kg(P>0.05).